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子囊菌交配型基因MAT1-2的系统发育研究
发表时间:2015-05-15 浏览量:2006 下载量:797
全部作者: | 贾冬梅,王雪亮*,史洋,张祥辉,张艳华,孙凤杰,潘洪玉 |
作者单位: | 吉林大学植物科学学院;佐治亚格威内特大学科学与技术学院 |
摘 要: | 以克隆获得的核盘菌交配型基因MAT1-2为“分子钟”,利用PAUP*软件对含有HMG-box交配型基因的79种子囊菌进行系统发育分析,通过核酸及氨基酸水平的分析,并与最新的Deep Hyphae(2006)分类系统进行比较研究。结果发现,利用MAT1-2所构建的系统进化树与传统分类所表现的进化关系基本一致,进一步证明了MAT1-2交配型基因在子囊菌系统发育过程中进化比较保守。该研究结果有助于对其他子囊菌交配型基因的克隆,以及子囊菌系统分类与进化的研究。同时,还可以利用MAT1-2交配型基因进行相关病害的分子监测,并为病害侵染循环与发生规律研究提供科学依据。 |
关 键 词: | 植物病理学;子囊菌;核盘菌;MAT1-2;系统分类 |
Title: | Research on phylogenetics of the mating type gene MAT1-2 in ascomycetes |
Author: | JIA Dongmei, WANG Xueliang*, SHI Yang, ZHANG Xianghui, ZHANG Yanhua, SUN Fengjie, PAN Hongyu |
Organization: | College of Plant Science, Jilin University; School of Science and Technology, Georgia Gwinnett College |
Abstract: | Based on the cloned Sclerotinia sclerotiorium mating type gene MAT1-2, this study generated a phylogenic analysis by PAUP* with other 79 mating type genes containing HMG-box domain from fungi. Through phylogenetics analysis on the level of nucleotide and amino acid and comparative study under the latest Deep Hyphae (2006), it is found that the evolutionary phylogenic tree constructed illustrates basically the same evolutionary relationship with that in traditional classification, and the function of mating type gene MAT1-2 is relatively conservative during the evolutionary process. The result is helpful to the study of cloning, phylogenetic classification and anagenesis of other mating type genes in ascomycetes. Moreover, it has great significance on the phylogeny, disease prediction and prevention caused by S. sclerotiorum. |
Key words: | phytopathology; ascomycota; Sclerotinia sclerotiorum; MAT1-2; phylogenetics |
发表期数: | 2015年5月第9期 |
引用格式: | 贾冬梅,王雪亮*,史洋,等. 子囊菌交配型基因MAT1-2的系统发育研究[J]. 中国科技论文在线精品论文,2015,8(9):892-898. |

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