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管花肉苁蓉幼苗自养阶段生长特性研究
发表时间：2015-05-15 浏览量：1654 下载量：483
| 全部作者： | 谢秋霖，赵敏，苗中芹，郭玉海 |
| 作者单位： | 中国农业大学农学与生物技术学院 |
| 摘 要： | 目的：明确管花肉苁蓉（Cistanche tubulosa）自养阶段芽管的生长速度、形态特征、膨压大小。方法：采用直接测量法测定芽管生长速度，显微镜观察法观察芽管形态特征，水势与渗透势差值法测膨压。结果：1）芽管生长呈“慢—快—慢”的趋势。芽管生长速度在露白后3 d时为0.10 mm·d^1，9 d时为0.60 mm·d^1，15 d时为0.05 mm·d^1，芽管最终长度5.0 mm. 2）芽管粗度呈“细—粗—细”的趋势。种子露白后3 d时芽管直径为0.15 mm，9 d时芽管直径为0.20 mm，15 d时芽管直径为0.15 mm. 3）芽管、吸器的膨压随其生长而呈逐渐增大的趋势。初期芽管的膨压为1.613 MPa，中期芽管的膨压为1.834 MPa，初生吸器的膨压为2.150 MPa. 结论：幼苗生长分为芽管缓慢伸长、快速伸长和吸器膨大3个阶段；随着芽管伸长、吸器膨大，其膨压显著提高。 |
| 关 键 词： | 作物生理学；生长特性；直接测量；管花肉苁蓉；膨压 |
| Title： | Study on the growth characteristics of Cistanche tubulosa seedling in autotrophy period |
| Author： | XIE Qiulin, ZHAO Min, MIAO Zhongqin, GUO Yuhai |
| Organization： | College of Agricultural and Biotechnology, China Agricultural University |
| Abstract： | Objective: The study is aimed at exploring the growth speed, morphological characteristics and turgor of Cistanche tubulosa seedling in autotrophy period. Methods: Growth speed of germ tube was measured directly, the morphological characteristics of germ tube was observed by using microscope, turgor was obtained by calculating the difference of water potential and osmotic potential. Results: 1) The growth trend of germ tube is “slow-fast-slow”, the growth speed of germ tube after radical breaking the peel 3, 9 and 15 days are 0.10, 0.60 and 0.05 mm·d^1, respectively and the length of germ tube reaches 5.0 mm eventually. 2) The thickness of germ tube is “thin-thick-thin” in growth, the diameters of germ tube are 0.15, 0.20 and 0.15 mm respectively 3, 9 and 15 days after radical breaking the peel. 3) The turgor of germ tube and haustorium is increased in growth. The turgor of germ tube in initial stage, metaphase and primary haustorium are 1.613, 1.834 and 2.150 MPa separately. Conclusion: The growth period of C. tubulosa seedling can be divided into three stages: slow elongation, fast elongation and haustorium swell. The turgor is increased significantly with germ tube elongation and haustorium swell. |
| Key words： | crop physiology; growth characteristics; direct measurement; Cistanche tubulosa; turgor |
| 发表期数： | 2015年5月第9期 |
| 引用格式： | 谢秋霖，赵敏，苗中芹，等. 管花肉苁蓉幼苗自养阶段生长特性研究[J]. 中国科技论文在线精品论文，2015，8（9）：907-914. |
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