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柑橘丛枝菌根真菌的分子鉴定
发表时间：2015-05-15 浏览量：1951 下载量：832
| 全部作者： | 宋放，王敏，潘志勇 |
| 作者单位： | 华中农业大学园艺林学学院，园艺植物生物学教育部重点实验室，农业部华中地区园艺作物生物学与种质创制重点实验室 |
| 摘 要： | 研究华中农业大学柑橘果园温州蜜柑枳砧根系样品及根际土壤样品的丛枝菌根群落组成。通过对根系样品DNA扩增菌根真菌18S SSU rRNA区段进行测序，获得120个来自丛枝菌根真菌（arbuscular mycorrhizal fungi, AMF）的序列。通过与MAARJAM数据库比对，获得92个有效序列，共鉴定到2个属（Glomus和Paraglomus）的8个种，其中Glomus属的Afrothismia foertheriana symbiont VT111占总序列数的85%. 此外，通过对土壤样品中的47个单孢子进行形态学观察和分子鉴定，获得5个属（Ambispora，Paraglomus，Glomus，Claroideoglomus和Archaeospora）的7个种，其中Ambispora属的Ambisporaceae Ambispora leptoticha VT242占总孢子数的75%，表明根系和土壤AMF优势菌种属分别为Glomus属和Ambispora属。研究初步鉴定出华中农业大学温州蜜柑枳砧根系及土壤中的AMF优势菌种属，为柑橘优势菌种的筛选和纯化奠定了基础。 |
| 关 键 词： | 园艺学；柑橘；丛枝菌根真菌；优势菌种 |
| Title： | Molecular identification of arbuscular mycorrhizal fungi in citrus |
| Author： | SONG Fang, WANG Min, PAN Zhiyong |
| Organization： | Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry Sciences, Huazhong Agricultural University |
| Abstract： | We investigated the arbuscular mycorrhizal fungal (AMF) community component in the roots and rhizosphere soils of citrus heterografts (mandarin orange as scion and Poncirus as rootstock) in the orchard located in Huazhong Agricultural University. Through the sequencing of AMF 18S SSU rRNA frangments amplified from roots DNA samples, we totally obtained 120 AMF sequences. Then we assigned these sequences against MAARJAM database, and a total of 92 effective sequences from 2 genus (Glomus and Paraglomus, corresponding to 8 species) were obtained. It is notable that 85% of all the 92 sequences were belonged to Glomus AMF Afrothismia foertheriana symbiont VT111 (species). In addition, a total of 47 spores from 5 genus (Ambispora, Paraglomus, Glomus, Claroideoglomus and Archaeospora, corresponding to 7 species) were isolated from rhizosphere soils based on morphology observation and molecular identification, 75% of which were belonged to Ambispora AMF Ambisporaceae Ambispora leptoticha VT242 (species). The results indicated that the dominant AMF in citrus roots and rhizosphere soils were belonged to Glomus and Ambispora, respectively. Our study preliminarily identified the dominant AMF genera in the roots and rhizosphere soils of citrus plants in Huazhong Agricultural University, which would provide a solid basis for the screening and purification of dominant AMF for citrus industry. |
| Key words： | horticulture; citrus; arbuscular mycorrhizal fungi; dominant bacteria |
| 发表期数： | 2015年5月第9期 |
| 引用格式： | 宋放，王敏，潘志勇. 柑橘丛枝菌根真菌的分子鉴定[J]. 中国科技论文在线精品论文，2015，8（9）：915-919. |
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