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枳水通道蛋白PIP基因家族成员的克隆及其在硼胁迫下的表达分析
发表时间：2015-05-15 浏览量：1959 下载量：887
| 全部作者： | 罗庆，颜廷帅，李峤虹，杨成泉，潘志勇，刘永忠，彭抒昂 |
| 作者单位： | 华中农业大学园艺植物生物学教育部重点实验室 |
| 摘 要： | 为探讨柑橘砧木枳[Poncirus trifoliate (L.) Raf.] PIP基因家族中响应硼胁迫的成员，从枳中克隆得到10个全长PIP基因家族成员的cDNA序列，包括3个PIP1s亚类基因和7个PIP2s亚类基因，并对克隆得到的序列进行生物信息学分析。结果表明：系统进化树上，PIP1s和PIP2s基因成员分别位于不同的2个大支上。除PtrPIP1;3，PtrPIP2;5及PtrPIP2;7仅含有4个跨膜区和1个NPA（Asn-Pro-Ala）特征基序，其他家族成员均含有6个跨膜区和2个NPA特征基序。通过实时定量PCR（real time-PCR，RT-PCR）分析家族成员在硼胁迫下时空表达模式，结果显示：PtrPIPs多在根中表达较高，其次是茎，仅有PtrPIP2;7成员在叶中高表达；在响应硼胁迫程度上，PtrPIP2s基因成员比PtrPIP1s基因成员要激烈得多。PtrPIP1s中PtrPIP1;2略响应硼胁迫，主要在茎中表达。PtrPIP2s中，PtrPIP2;2在缺硼处理下，根的表达量最高；而PtrPIP2;3在硼毒害处理下，根的表达量最高；PtrPIP2;4在硼胁迫下，在茎中的表达量最高，且与组织特异性一致；PtrPIP2;7在缺硼处理下茎的表达量最高，在硼毒害处理下叶的表达量最高。因此，推测PtrPIPs基因成员可能参与了硼胁迫响应过程，为进一步阐明柑橘硼胁迫响应的分子机制提供了理论依据。 |
| 关 键 词： | 果树学；枳；PtrPIPs；基因家族；硼胁迫；实时定量PCR |
| Title： | Cloning of aquaporins PIP gene family from Poncirus trifoliata (L.) Raf. and expression under boron stress |
| Author： | LUO Qing, YAN Tingshuai, LI Qiaohong, YANG Chengquan, PAN Zhiyong, LIU Yongzhong, PENG Shu’ang |
| Organization： | Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University |
| Abstract： | In order to study the relationship of aquaporins PIP gene family and boron transportation in trifoliate orange [Poncirus trifoliata (L.) Raf.], ten numbers of PIP subfamily gene were cloned which included three PtrPIP1s subgenes and seven PtrPIP2s subgenes and analyzed by using bioinformatics. We found that PtrPIP1s and PtrPIP2s genes were located at two different large branches in phylogenetic tree, and their amino acid sequences were consisted of six transmembrances and two conserved NPA (Asn-Pro-Ala) motifs while PtrPIP1;3, PtrPIP2;5 and PtrPIP2;7 gene consisted of four transmembrances and one conserved NPA motif. The real time-PCR (RT-PCR) analysis of PtrPIPs gene family in different organs under boron stress were studied, which responded mainly in root, then in stem and leaf (only PtrPIP2;7), respectively. All PtrPIP2s genes responded strongly to boron stress whereas PtrPIP1s responded slightly to it. The relative expression levels of PtrPIP1;2 genes were mainly in stem among PtrPIP1s genes. Among PtrPIP2s genes, the highest relative expression of PtrPIP2;2 and PtrPIP2;3 gene were in root under boron deficiency and high boron stress, respectively. The PtrPIP2;4 gene was in stem under boron stress, which was in accordance with its organ specificity. The highest relative expression of PtrPIP2;7 gene was in leaf under high boron stress, which was in stem under boron deficiency. So we conclude that the members of PtrPIPs gene family maybe participate in the process of responding to boron stress, which provides theoretical basis for further clarifying the molecular mechanism of responding to boron stress in citrus. |
| Key words： | pomology; Poncirus trifoliate (L.) Raf.; PtrPIPs; gene family; boron stress; real time-PCR |
| 发表期数： | 2015年5月第9期 |
| 引用格式： | 罗庆，颜廷帅，李峤虹，等. 枳水通道蛋白PIP基因家族成员的克隆及其在硼胁迫下的表达分析[J]. 中国科技论文在线精品论文，2015，8（9）：920-932. |
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