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激光扫描共聚焦显微镜在生物医学中的应用

发表时间:2015-06-15  浏览量:2548  下载量:1597
全部作者: 严意炜,吴小玲,段磊,王伟,朱松盛,刘宾
作者单位: 南京医科大学基础医学院
摘 要: 激光扫描共聚焦显微镜(confocal laser scanning microscope,CLSM)能有效排除非焦面的光线干扰,并结合激光和计算机图像处理技术,提高光学显微镜的分辨率,能够无损伤地观察细胞、组织的二维、三维图像,是近代发展起来的一项具有跨时代意义的高科技新产品。这里从历史、原理及结构、成像相关因素等多个角度介绍了CLSM,并综述了其在生物医学中的一些应用。
关 键 词: 生物医学工程学;激光扫描共聚焦显微镜;综述;生物医学;图像处理
Title: Certain application of confocal laser scanning microscopy in biomedical engineering
Author: YAN Yiwei, WU Xiaoling, DUAN Lei, WANG Wei, ZHU Songsheng, LIU Bin
Organization: School of Basic Medical Sciences, Nanjing Medical University
Abstract: Confocal laser scanning microscope (CLSM) can eliminate the focal plane of the light interference effectively and connect with the laser and computer image processing technology to improve the resolution of the optical microscope. It can also observe the two-dimension or three-dimension images of cells and tissues without any damage. It is a modern developed epochal significance of high-tech new products. This article introduced the CLSM from the four aspects of the history, principle and structure and imaging related factors, and reviewed its biological applications.
Key words: biomedical engineering; confocal laser scanning microscope; review; biomedicine; image processing
发表期数: 2015年6月第11期
引用格式: 严意炜,吴小玲,段磊,等. 激光扫描共聚焦显微镜在生物医学中的应用[J]. 中国科技论文在线精品论文,2015,8(11):1103-1110.
 
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