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温阳消饮法对胸腔积液大鼠壁层胸膜基因表达影响的实验研究

发表时间:2015-06-15  浏览量:1731  下载量:939
全部作者: 赖宇,赵欣悦,李思思,蒋萃,肖桦,张琦
作者单位: 成都中医药大学基础医学院;苏州市吴中区横泾卫生院;成都中医药大学附属医院针灸学校龙泉校区教务科
摘 要: 目的:利用基因芯片技术,分析温阳消饮法对胸腔积液大鼠壁层胸膜基因表达的调节作用。方法:48只雄性SD大鼠适应性饲养3 d后,随机分为A组(40只)与空白对照组(8只)。A组动物以戊巴比妥钠麻醉后,采用1% λ-角叉菜胶(λ-carrageenan)溶液进行右侧胸膜腔注射。24 h后,运用数字成像系统观察胸部X光片,筛选出胸腔积液明显的大鼠作为造模成功的动物备用。将造模成功的动物随机分为模型组(9只),每日1次生理盐水灌胃(5 mL/kg);温阳消饮组(10只),每日1次温阳消饮法代表方水煎液灌胃(5 mL/kg);空白对照组(8只),每日1次生理盐水灌胃(5 mL/kg)。连续给药4 d后,动物通过在水合氯醛深度麻醉下从腹主动脉流血致安乐死。每组取3只动物的右侧壁层胸膜,利用Agilent单通道表达谱芯片分析胸膜组织基因表达情况。结果:模型组与空白对照组、温阳消饮组与模型组差异表达基因涉及多个生物学过程。在造模因素作用下,基因Fgd4, Mef2a,Fhl3,Dyrk2,Slc24a6,Pbrm1,LOC498368,Tp53i11,Rcan3,Hapln4,Abi1和Hlx表达上调,在中药干预下表达下调;Nme7,Pold2,Klk1l和Fkbp5在造模条件下表达下调,用药后上调。结论:温阳消饮法有助于λ-角叉菜胶诱导的豚鼠胸腔积液的吸收,其机制可能与温阳消饮法下调壁层胸膜炎症促进基因Mef2a,凋亡诱导基因Dyrk2和Tp53i11表达,进而改善炎症效应有关。
关 键 词: 分子药理学;温阳消饮法;基因芯片技术;胸腔积液
Title: An experimental study on the effect of therapy of warming Yang to relieve fluid retention on the gene expression in parietal pleura of r at with hydrothorax
Author: LAI Yu, ZHAO Xinyue, LI Sisi, JIANG Cui, XIAO Hua, ZHANG Qi
Organization: School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine; Hengjing Hospital of Wuzhong District; Academic Affairs Section, Longquan Campus of Acupuncture School, Teaching Hospital of Chengdu University of T. C. M
Abstract: Objective: Genechip technology was used to measure changes in gene expression, affected by therapeutic method of warming Yang to relieve fluid retention of parietal pleura in rat. Methods: 48 male SD rats were allowed to acclimate for 3 d before used, and then were randomly divided into group A (40 rats) and control group (8 rats). Rats in group A were anesthetized with pentobarbital sodium, followed by the right intrapleural cavity injection of 1% λ-carrageenan solution. 24 h later, after the injection with carrageenan, the chest of rats was X-rayed, and the animals with a significant pleural effusion were determined by computed radiography for further use as modeling rats. These valid rats were further divided into model group (9 rats) with saline (5 mL/kg, 1 time per day), treatment group (10 rats) with intragastric administration of Chinese herb decoction (5 mL/kg, 1 time per day) as well as control group (8 rats) which were given saline (5 mL/kg, 1 time per day). Four days later, the animals were euthanized by bleeding from the abdominal aorta under deep anesthesia with chloral hydrate. The right parietal pleura of three rats from each group were obtained and the differential gene expression was analyzed using single channel Agilent genechip. Results: Several biological processes are involved in the differential gen expression by comparing the model group and control group as well as the treatment group and model group. The therapy of warming Yang to relieve fluid retention could conversely regulate differential expression of 16 genes caused by λ-carrageenan. The expressions of Fgd4, Mef2a, Fhl3, Dyrk2, Slc24a6, Pbrm1, LOC498368, Tp53i11, Rcan3, Hapln4, Abi1and Hlx are increased under modeling effect while decreased after intervened by herb; the expressions of Nme7, Pold2, Klk1l and Fkbp5 act oppositely. Conclusion: The therapy of warming Yang can relieve fluid retention caused by λ-carrageen, which probably related to the up-regulation of Mef2a and down-regulation of Dyrk2 and Tp53i11 expressison in parietal pleura and then reduce inflammation.
Key words: molecular pharmacology; therapy of warming Yang; genechip technology; pleural effusion
发表期数: 2015年6月第11期
引用格式: 赖宇,赵欣悦,李思思,等. 温阳消饮法对胸腔积液大鼠壁层胸膜基因表达影响的实验研究[J]. 中国科技论文在线精品论文,2015,8(11):1164-1172.
 
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