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5-HT1D受体激活对小鼠小直径三叉神经节神经元A型K+电流的影响

发表时间:2015-06-15  浏览量:2623  下载量:1000
全部作者: 张怡,陶金
作者单位: 苏州大学医学部基础医学与生物科学学院
摘 要: 目的:研究5-HT1D受体的激活对小鼠小直径三叉神经节(trigeminal ganglion,TG)神经元A型钾离子电流的影响。方法:培养小鼠TG神经元细胞,利用全细胞膜片钳技术研究5-HT1D受体的激活对小直径TG神经元A型钾电流作用,并通过药理学方法进一步研究其信号通路作用机制。结果:1)电生理研究表明,治疗偏头痛的药物舒马曲坦(sumatriptan)对小直径TG神经元细胞上的A型钾离子电流具有增强作用;2)5-HT1D受体的激活对小直径TG神经元细胞上的A型钾离子电流的增强作用具有剂量依赖性。10 nmol "L"1 L694247(5-HT1D受体的激动剂)对A型钾电流的增长率约为67.8%,5-HT1B受体的激活对小直径TG神经元细胞上的A型钾离子电流不具有增强作用;3)在信号通路方面,蛋白激酶C(PKC)的选择性阻断剂GF109203X白屈菜赤碱(chelerythrine chloride)无法阻断L694247对A型钾电流的增强作用。结论:L694247可通过作用于小鼠小直径TG神经元上的5-HT1D受体来增强A型钾电流;PKC信号通路不参与5-HT1D受体对A型钾电流的增强作用。
关 键 词: 基础医学其他学科;5-HT1D受体;A型钾电流;三叉神经节神经元;偏头痛;舒马曲坦
Title: Effects of A-type K+ currents by the 5-HT1D receptor activation involving migraine in small trigeminal ganglion neurons in mice
Author: ZHANG Yi, TAO Jin
Organization: School of Biology & Basic Medical Sciences, Medical College of Soochow University
Abstract: Objective: To investigate the activation of 5-HT1D receptors affecting the A-type K+ currents in trigeminal ganglion (TG) neurons and its possible mechanisms. Methods: By cultivating TG neurons in mice, the whole-cell patch clamp recording was used to investigate the effect of 5-HT1D receptor on A-type K+ currents in minor diameter TG neurons. Pharmacological methods were used to further explore its signaling pathways. Results: 1) Electrophysiology results reveal that sumatriptan used for treating migraine increases A-type K+ currents in mice’s minor diameter TG neurons; 2) The activation of 5-HT1D receptors increases A-type K+ currents in a dose-dependent manner in mice’s minor diameter TG neurons. 10 nmol"L"1 L694247 (agonist of 5-HT1D receptor) increases A-type K+ currents by about 67.8%. The activation of 5-HT1B receptors doesn’t increase A-type K+ currents in mice’s minor diameter TG neurons; 3) In signal pathway, GF109203X (chelerythrine chloride), the selective blocker of protein kinase C (PKC) pathway, was not involved in the 5-HT1D receptor-mediated effect. Conclusion: L694247 can activate 5-HT1D receptors in minor-diameter TG neurons to increase A-type K+ currents, while PKC pathway is not involved in the 5-HT1D receptor-mediated effect.
Key words: other subjects of basic medicine; 5-HT1D receptors; A-type K+ currents; trigeminal ganglion neurons; migraine; sumatriptan
发表期数: 2015年6月第11期
引用格式: 张怡,陶金. 5-HT1D受体激活对小鼠小直径三叉神经节神经元A型K+电流的影响[J]. 中国科技论文在线精品论文,2015,8(11):1173-1180.
 
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