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锰致小鼠黑质-纹状体神经功能损伤的实验研究

发表时间:2015-06-15  浏览量:1779  下载量:592
全部作者: 宋奇繁,邓宇,徐斌,王璨,刘巍,徐兆发
作者单位: 中国医科大学公共卫生学院,环境卫生学教研室
摘 要: 目的:探讨锰对小鼠黑质-纹状体神经功能的影响,为阐明锰中毒的发病机制和防护作用提供依据。方法:小鼠48只,均分为4组。第1组为对照组;第2~4组分别为低、中、高剂量染锰组。第1组腹腔注射0.9% NaCl,第2~4组腹腔注射12.5,25.0,50.0 mg/kg MnCl2. 每天染毒1次,持续2周。最后一次处理完后,检测小鼠的自主活动次数、站立次数和转棒潜伏期秒数。观察Morris水迷宫实验逃避潜伏期、游泳游程和穿环次数。检测避暗实验24 h前后潜伏期和进暗室次数。HE染色和透射电镜观察黑质和纹状体组织形态及超微结构。结果:与对照组比较,随着锰浓度的升高,小鼠的自主活动次数、站立次数和转棒潜伏期秒数逐渐减少;水迷宫实验逃避潜伏期、游泳距离逐渐增加,6 d和13 d穿环次数逐渐减少;避暗实验24 h后潜伏期逐渐降低,其进入暗室次数逐渐增加。随着染锰剂量的增加,黑质和纹状体组织形态和超微结构损伤逐渐加重。结论:过量的锰暴露可致小鼠运动与学习记忆障碍,其机制可能与锰所致的黑质�纹状体神经系统受损有关。
关 键 词: 环境医学;锰;黑质;纹状体;运动障碍;学习记忆障碍
Title: Study on injury of neural function in substantia nigra and striatum of mice caused by manganese
Author: SONG Qifan, DENG Yu, XU Bin, WANG Can, LIU Wei, XU Zhaofa
Organization: Department of Environmental Health, School of Public Health, China Medical University
Abstract: Objective: To explore manganese (Mn) induced injury of neural function of substantia nigra and striatum and to elucidate the pathogenesis of manganese poisoning and provide the basis for protective effect. Methods: 48 mice were randomly divided into four groups. The first to fourth groups were the control, low Mn, medium Mn and high Mn groups, respectively. The mice in the first to fourth groups were i.p. injected with 0.9% NaCl, 12.5, 25.0, and 50.0 mg/kg MnCl2, respectively, which were exposed and intervened once every day for 2 weeks. After that, mouse independent activity frequency, stand-up times and rotating bar latency were detected. The Morris water maze test escape latency, swimming distance and frequency of crossing the loop were increased. Dark avoidance test before and after 24 h incubation period and into the darkroom times were also detected. HE staining was used to observe the morphological changes of substantia nigra and striatum and transmission electron microscope was used to observe ultrastructural changes of substantia nigra and striatum. Results: Compared with the control group, as the elevation of the concentration of MnCl2 administration, mice independent activity frequency, the number of standing and rotating bar latencies are reduced significantly. The Morris water maze test escape latency and swimming distance are gradually increased. Frequency of crossing the loop are gradually decreased after 6 days and 13 days. Entering the darkroom latency through test in mice are decreased gradually after 24 h incubation period, and entering the darkroom times gradually increased after 24 h incubation period. It is also found that with the increase of Mn concentration, the substantia nigra and striatum tissue pathological and ultrastructural injury are aggravated obviously. Conclusion: Excessive Mn exposure can cause dyskinesia of mice and the impairment of learning and memory, which may be related to the substantia nigra and striatum injury caused by Mn.
Key words: environmental medicine; manganese; substantia nigra; striatum; dyskinesia; learning and memory impairment
发表期数: 2015年6月第11期
引用格式: 宋奇繁,邓宇,徐斌,等. 锰致小鼠黑质-纹状体神经功能损伤的实验研究[J]. 中国科技论文在线精品论文,2015,8(11):1181-1188.
 
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