您的位置:首页  > 论文页面

蝎毒多肽干预慢性粒细胞白血病小鼠模型P210及JAK1表达

发表时间:2015-09-15  浏览量:1416  下载量:416
全部作者: 郝征,杨文华
作者单位: 天津中医药大学中医学院;天津中医药大学第一附属医院血液科
摘 要: 目的:观察蝎毒多肽(peptide extract from scorpion venom,PESV)干预NOD/SCID小鼠模型体内慢性粒细胞白血病(chronic myelocytic leukemia,CML)P210,JAK1表达水平变化。方法:应用K562细胞注入经过铯-137源照射的NOD/SCID小鼠体内,建立CML NOD/SCID小鼠模型;对实验小鼠随机分组,用Western blotting检测PESV治疗后小鼠体内融合蛋白P210表达水平,ELISA法检测小鼠JAK1表达水平。结果:PESV能够降低模型小鼠体内融合蛋白P210表达水平,抑制JAK1过度表达,其抑制效果与PESV浓度具有相关性。结论:PESV通过干预CML 融合蛋白P210及JAK1表达,有效地阻断了 CML传变进展,揭示了PESV阻抑CML传变的微观机理。
关 键 词: 中医内科学;蝎毒多肽;慢性粒细胞白血病;P210;JAK1
Title: Influence of peptide extracts from scorpion venom on P210 and JAK1 expression in chronic myelocytic leukemia mice
Author: HAO Zheng, YANG Wenhua
Organization: School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine; Department of Hematology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Abstract: Objective: To investigate the peptide extract from scorpion venom (PESV) producing effects on P210, JAK1 expression in chronic myelocytic leukemia (CML)-NOD/SCID mice. Methods:K562 cells were injected into NOD/SCID mice, and the mice were irradiated on body by 137-Cs beforehand in order to establish the animal model of CML-NOD/SCID mice. Then, we randomly divided the mice of model establishment into several groups, and we used Western blotting method to examine P210 expression in mice, and simultaneously used ELISA method to examine JAK1 expression. Results: PESV could suppress P210 expression and inhibit JAK1 expression, the inhibiting effect depends on PESV concentration. Conclusion: PESV can inhibit CML cell transmutation by influencing the expression of P210 and JAK1, which reveals the microscopic mechanism on PESV inhibiting CML transmutation.
Key words: internal medicine of traditional Chinese medicine; peptide extract from scorpion venom; chronic myelocytic leukemia; P210; JAK1
发表期数: 2015年9月第17期
引用格式: 郝征,杨文华. 蝎毒多肽干预慢性粒细胞白血病小鼠模型P210及JAK1表达[J]. 中国科技论文在线精品论文,2015,8(17):1776-1780.
 
0 评论数 0
暂无评论
友情链接