您的位置:首页  > 论文页面

裸鼠阳虚证乳腺癌骨转移模型的建立

发表时间:2015-09-15  浏览量:1476  下载量:539
全部作者: 黄立中,毛丹,冯磊,张慧
作者单位: 湖南中医药大学中西医结合学院; 中南大学湘雅二医院中医科
摘 要: 目的:采用病、证复合模型的方法建立裸鼠阳虚证乳腺癌骨转移模型。方法:采用臀部肌肉注射氢化可的松的方法建立裸鼠阳虚证模型;采用左心室注射乳腺癌细胞MDA-MB-231悬液建立裸鼠乳腺癌骨转移模型;动态观察各组裸鼠生存情况、体重变化,进行X射线摄像、影像学评分、病理组织观察,测定环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)及甲状腺激素T3等值。结果:肌注氢化可的松后,裸鼠出现畏寒肢冷、活动减少、精神萎靡、皮毛少光泽、饮食减少、大便清稀等阳虚表现,且cAMP明显下降,cGMP明显升高,cAMP/cGMP值下降,T3值明显升高,左心室注射乳腺癌细胞悬液后裸鼠出现不同程度的骨转移,且经X射线摄像检查和病理组织学检查确诊。结论:1)对裸鼠肌注氢化可的松可成功建立阳虚证模型;2)左心室注射法建立乳腺癌骨转移模型的成功率较高;3)阳虚证动物模型更容易发生骨转移。
关 键 词: 中西医结合医学;乳腺癌;骨转移;阳虚证;裸鼠模型
Title: Establishment on Yang deficiency and bone metastasis model of nude mice
Author: HUANG Lizhong, MAO Dan, FENG Lei, ZHANG Hui
Organization: School of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medcine; Department of Traditional Chinese Medicine, The Second Xiangya Hospital of Central South University
Abstract: Objective: To establish a Yang deficiency and bone metastasis model of nude mice by combining disease and syndrome method. Methods: We used intramuscular injected hydrocortisone for nude mice to establish the model Yang deficiency syndrome. The bone metastasis model for nude mice was established by injecting breast cancer MDA-MB-231 cells suspension to the ventriculus sinistee. In addition, the nude mice survival and the body weight were observed and the X-ray imaging, the imaging score and pathology observation were conducted and the value of cAMP, cGMP and thyroid hormone T3 were determined. Results: After injecting hydrocortisone, the nude mice had Yang deficiency performance, such as aversion to cold, reduced activity, apathetic, fur less shiny, eating less and thin stool, as well as cAMP decreasing while cGMP increasing, cAMP/cGMP decreasing and T3 increasing obviously. After injecting breast cancer cells suspension to the ventriculus sinistee, the nude mice appeared different degree bone metastasis, and the X-ray imaging examination and histopathological examination confirmed the diagnosis. Conclusion: 1) Hydrocortisone injection can establish the Yang deficiency model of nude mice successfully. 2) Injecting breast cancer cells suspension to the ventriculus sinistee can establish the bone metastasis model of breast cancer in a high successful rate. 3) Animal model of Yang deficiency is more likely to occur with bone metastasis.
Key words: integrated traditional Chinese and western medicine; breast cancer; bone metastasis; Yang deficiency syndrome; nude mouse model
发表期数: 2015年9月第17期
引用格式: 黄立中,毛丹,冯磊,等. 裸鼠阳虚证乳腺癌骨转移模型的建立[J]. 中国科技论文在线精品论文,2015,8(17):1809-1817.
 
0 评论数 0
暂无评论
友情链接