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菌草灵芝菌糟提取物对免疫力低下小鼠血清中IL-1β,TNF-α和IFN-γ含量的影响

发表时间:2015-10-15  浏览量:1248  下载量:338
全部作者: 刘艳玲,罗海凌,林冬梅,林兴生,林占q
作者单位: 福建农林大学生命科学学院;国家菌草工程技术研究中心
摘 要: 在菌草灵芝菌糟提取物(hot water extract of spent mushroom substrate from Ganoderma lucidum cultivated by JUNCAO,简记为HWE)可以提高正常小鼠免疫功能的基础上,研究HWE对环磷酰胺致免疫力低下小鼠模型血清中IL-1β,TNF-α和IFN-γ的影响。空白对照组(记为CK1),正常对照组(记为CK2),环磷酰胺模型组(记为CY),CY+低剂量HWE组(记为CL),CY+中剂量HWE组(记为CM),CY+高剂量HWE组(记为CH)小鼠灌胃提取物的剂量分别为0,1.0,0,0.5,1.0,2.0 g/kg BW. 结果发现:与CY组相比,CK1,CK2组血清中IL-1β含量明显降低(P < 0.01);CL,CM和CH组小鼠血清中TNF-α比CY组的浓度低,但差异不显著(P>0.05);CL,CM和CH组小鼠血清中的IFN-γ明显低于CY组(P<0.01)。结果表明:HWE能提高小鼠的脾脏指数和胸腺指数,可抑制血清中IL-1β,IFN-γ,TNF-α等细胞因子的含量。
关 键 词: 微生物学;菌草灵芝菌糟提取物;环磷酰胺;IL-1β;TNF-α;IFN-γ
Title: Effects of hot water extract of spent mushroom substrate from Ganoderma lucidum cultivated by JUNCAO on IL-1β, TNF-α and IFN-γ in serum of immune-deficient mice
Author: LIU Yanling, LUO Hailing, LIN Dongmei, LIN Xingsheng, LIN Zhanxi
Organization: College of Life Science, Fujian Agriculture and Forestry University; China National Engineering Research Center of JUNCAO Technology
Abstract: This experiment studied the immunity in immune-deficient mice by cyclophosphamidum based on that hot water extract of spent mushroom substrate from Ganoderma lucidum cultivated by JUNCAO (abbreviated as HWE) can enhance the immune function in normal mice by measuring the concentrations of IL-1β, IFN-γ, TNF-α in serum. And the HWE dose on the mice the groups of blank control group (CK1), control group (CK2), cyclophosphamidum model (CY), CY+low HWE (CL), CY+medium HWE (CM), CY+high HWE (CH) were 0, 1.0, 0, 0.5, 1.0, 2.0 g/kg BW, respectively. The experiment results showed that, compared to CY, IL-1β in serum of CK1 and CK2 were significantly decreased (P<0.01), and TNF-α in serum of CL, CM, CH were decreased, where there was no significant difference (P>0.05), IFN-γ in serum of CL, CM, CH were markedly decreased compared to CK1 (P<0.01). And the results indicate that HWE can enhance the spleen index and thymus index and inhibit the concentration of IL-1β, TNF-α and IFN-γ in serum.
Key words: microbiology; hot water extract of spent mushroom substrate from Ganoderma lucidum cultivated by JUNCAO; cyclophosphamidum; IL-1β; TNF-α; IFN-γ
发表期数: 2015年10月第19期
引用格式: 刘艳玲,罗海凌,林冬梅,等. 菌草灵芝菌糟提取物对免疫力低下小鼠血清中IL-1β,TNF-α和IFN-γ含量的影响[J]. 中国科技论文在线精品论文,2015,8(19):1995-2001.
 
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