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大鼠骨髓间充质干细胞条件培养基通过ERK1/2促进肌腱细胞增殖

发表时间:2015-10-15  浏览量:1458  下载量:614
全部作者: 梁青飞,罗庆,杨先炯,宋关斌
作者单位: 重庆大学生物工程学院;贵州医科大学化学教研室
摘 要: 目的:探讨大鼠骨髓间充质干细胞条件培养基(conditioned medium from rat bone marrow derived mesenchymal stem cell,rMSC-CM)对肌腱细胞增殖的影响及相关机理。方法:原代分离培养大鼠骨髓间充质干细胞(rat bone marrow derived mesenchymal stem cells,rMSCs)和肌腱细胞,90%融合的rMSCs无血清培养24 h收集上清,为rMSC-CM,细胞计数法考察rMSC-CM对肌腱细胞增殖的影响,Western blotting和PD98059考察ERK1/2(extracellular regulated protein kinases 1/2)磷酸化在rMSC-CM促肌腱细胞增殖中的作用。结果:rMSC-CM处理24 h能显著促进肌腱细胞增殖,且在rMSC-CM处理15 min后ERK1/2被显著激活。采用PD98059抑制rMSC-CM对肌腱细胞ERK1/2的激活,rMSC-CM对肌腱细胞增殖的促进作用也受到抑制。结论:rMSC-CM可通过激活ERK1/2促进肌腱细胞增殖。
关 键 词: 分子生物学;骨髓间充质干细胞;条件培养基;肌腱细胞;增殖;ERK1/2
Title: Proliferation promotion of tenocytes through ERK1/2 on conditioned medium from rat bone marrow derived
Author: LIANG Qingfei, LUO Qing, YANG Xianjiong, SONG Guanbin
Organization: College of Bioengineering, Chongqing University; Department of Chemistry, Guizhou Medical University
Abstract: Objective: To study the effect of conditioned medium from rat bone marrow derived mesenchymal stem cells (rMSC-CM) on the proliferation of rat tenocytes and the related mechanisms. Methods: Rat bone marrow derived mesenchymal stem cells (rMSCs) and tenocytes were primary cultured; 90% rMSCs fusion were cultured for 24 h in serum free medium and the supernatant was collected as rMSC-CM. Cytometry was performed to detect the proliferation of tenocytes. Western blotting and PD98059, the inhibitor of extracellular regulated protein kinases 1/2 (ERK1/2), were used to investigate the effect of ERK1/2 on the rMSC-CM-induced tenocyte proliferation. Results: Western blotting showed that rMSC-CM treatment of 24 h can significantly promote the proliferation of tenocytes and the phosphorylation of ERK1/2 in tenocytes was promoted by 15 min of rMSC-CM treatment. PD98059 blocked the rMSC-CM-activated ERK1/2 and suppressed the rMSC-CM-promoted tenocyte proliferation. Conclusion: rMSC-CM promotes tenocyte proliferation through the activation of ERK1/2.
Key words: molecular biology; bone marrow derived mesenchymal stem cells; conditioned medium; tenocytes; proliferation; extracellular regulated protein kinases 1/2
发表期数: 2015年10月第19期
引用格式: 梁青飞,罗庆,杨先炯,等. 大鼠骨髓间充质干细胞条件培养基通过ERK1/2促进肌腱细胞增殖[J]. 中国科技论文在线精品论文,2015,8(19):2014-2020.
 
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