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大鼠骨髓间充质干细胞条件培养基通过ERK1/2促进肌腱细胞增殖
发表时间：2015-10-15 浏览量：1954 下载量：894
| 全部作者： | 梁青飞，罗庆，杨先炯，宋关斌 |
| 作者单位： | 重庆大学生物工程学院；贵州医科大学化学教研室 |
| 摘 要： | 目的：探讨大鼠骨髓间充质干细胞条件培养基（conditioned medium from rat bone marrow derived mesenchymal stem cell，rMSC-CM）对肌腱细胞增殖的影响及相关机理。方法：原代分离培养大鼠骨髓间充质干细胞（rat bone marrow derived mesenchymal stem cells，rMSCs）和肌腱细胞，90%融合的rMSCs无血清培养24 h收集上清，为rMSC-CM，细胞计数法考察rMSC-CM对肌腱细胞增殖的影响，Western blotting和PD98059考察ERK1/2（extracellular regulated protein kinases 1/2）磷酸化在rMSC-CM促肌腱细胞增殖中的作用。结果：rMSC-CM处理24 h能显著促进肌腱细胞增殖，且在rMSC-CM处理15 min后ERK1/2被显著激活。采用PD98059抑制rMSC-CM对肌腱细胞ERK1/2的激活，rMSC-CM对肌腱细胞增殖的促进作用也受到抑制。结论：rMSC-CM可通过激活ERK1/2促进肌腱细胞增殖。 |
| 关 键 词： | 分子生物学；骨髓间充质干细胞；条件培养基；肌腱细胞；增殖；ERK1/2 |
| Title： | Proliferation promotion of tenocytes through ERK1/2 on conditioned medium from rat bone marrow derived |
| Author： | LIANG Qingfei, LUO Qing, YANG Xianjiong, SONG Guanbin |
| Organization： | College of Bioengineering, Chongqing University; Department of Chemistry, Guizhou Medical University |
| Abstract： | Objective: To study the effect of conditioned medium from rat bone marrow derived mesenchymal stem cells (rMSC-CM) on the proliferation of rat tenocytes and the related mechanisms. Methods: Rat bone marrow derived mesenchymal stem cells (rMSCs) and tenocytes were primary cultured; 90% rMSCs fusion were cultured for 24 h in serum free medium and the supernatant was collected as rMSC-CM. Cytometry was performed to detect the proliferation of tenocytes. Western blotting and PD98059, the inhibitor of extracellular regulated protein kinases 1/2 (ERK1/2), were used to investigate the effect of ERK1/2 on the rMSC-CM-induced tenocyte proliferation. Results: Western blotting showed that rMSC-CM treatment of 24 h can significantly promote the proliferation of tenocytes and the phosphorylation of ERK1/2 in tenocytes was promoted by 15 min of rMSC-CM treatment. PD98059 blocked the rMSC-CM-activated ERK1/2 and suppressed the rMSC-CM-promoted tenocyte proliferation. Conclusion: rMSC-CM promotes tenocyte proliferation through the activation of ERK1/2. |
| Key words： | molecular biology; bone marrow derived mesenchymal stem cells; conditioned medium; tenocytes; proliferation; extracellular regulated protein kinases 1/2 |
| 发表期数： | 2015年10月第19期 |
| 引用格式： | 梁青飞，罗庆，杨先炯，等. 大鼠骨髓间充质干细胞条件培养基通过ERK1/2促进肌腱细胞增殖[J]. 中国科技论文在线精品论文，2015，8（19）：2014-2020. |
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