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不同造模时间帕金森大鼠旋转行为的变化

发表时间:2015-10-15  浏览量:1440  下载量:477
全部作者: 曲庆洋,谢金鹿,耿希文,李敏,杨茂全,王琳涵,王敏
作者单位: 山东师范大学生命科学学院;山东师范大学管理科学与工程学院
摘 要: 目的:进一步探究6-羟基多巴胺法制作帕金森病(Parkinson’s disease,PD)大鼠模型,为鉴定PD大鼠模型提供指导。方法:以Wistar雄性大鼠为研究对象,中脑腹侧被盖区(ventral tegmental area,VTA)、黑质致密部(substantia nigra pars compacta,SNc)偏侧2位点联合注射6-羟基多巴胺制作PD大鼠模型,手术不同时间后,颈部肌肉注射盐酸阿朴吗啡(APO)诱导旋转,观察旋转行为的变化,并辅助组织学观察以验证旋转实验结果。结果:手术后2周内,6只大鼠双向旋转,SNc神经元变性不显著;手术3周后,6只大鼠中有2只大鼠造模成功,成功率为33.33%;手术4周后,6只大鼠中有5只大鼠造模成功,成功率为83.33%.成功的PD大鼠损伤侧脑SNc多巴胺能神经元数量显著减少,细胞核与细胞质的完整性遭到破坏,胶质细胞增生明显。结论:VTA,SNc偏侧2位点联合注射6-羟基多巴胺法是制作PD大鼠模型的有效方法,手术4周后,造模成功率最高。
关 键 词: 神经生理学;帕金森病;运动障碍;6-羟基多巴胺
Title: Changes of rotation behavior of mice with Parkinson’s disease in different modeling time
Author: QU Qingyang, XIE Jinlu, GENG Xiwen, LI Min, YANG Maoquan, WANG Linhan, WANG Min
Organization: School of Life Science, Shandong Normal University; School of Mangagement Science and Engineering, Shandong Normal University
Abstract: Objective: To further explore the method of making a Parkinson’s disease (PD) rat model by injecting 6-hydroxydopamine and to provide guidance for identifying the PD rat model. Methods: We took male Wistar rats as the object of study. Both the ventral tegmental area (VTA) and the substantia nigra pars compacta (SNc) were injected with 6-hydroxydopamine to make a PD rat model. Then neck muscles were injected with apomophine to induce rotation and the change of rotation behavior, which was observed after different surgery time. At the same time, histological was observed to verify rotation experiment results. Results: Within two weeks, all rats rotated in both directions and the degeneration of neurons which located in the rats lesioned brain SNc was not significant. After three weeks, two out of six rats were made model successfully, the success rate was 33.33%. After four weeks, five out of six rats were made model successfully, the success rate was 83.33%. The number of dopaminergic neuron which located in the PD rats lesioned brain SNc was reduced significantly. The integrity of the nucleus and cytoplasm were destroyed. The glial cells showed obvious hyperplasia. Conclusion: The above mentioned method was effective to make a PD rat model. The success rate was the highest after four weeks.
Key words: neurophysiology; Parkinson’s disease; movement disorder; 6-hydroxydopamine
发表期数: 2015年10月第19期
引用格式: 曲庆洋,谢金鹿,耿希文,等. 不同造模时间帕金森大鼠旋转行为的变化[J]. 中国科技论文在线精品论文,2015,8(19):2027-2033.
 
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