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小菜蛾肽聚糖识别蛋白的生物信息学分析

发表时间:2015-11-15  浏览量:1612  下载量:476
全部作者: 梁昊,黄斌,侯有明
作者单位: 福建农林大学植物保护学院
摘 要: 为深入研究小菜蛾(Plutella xylostella L.)中肽聚糖识别蛋白(peptidoglycan recognition protein,PGRP)的具体功能,采用生物信息学方法对小菜蛾基因组中PGRP基因编码蛋白从氨基酸理化性质、亚细胞定位、保守结构域、高级结构及酰胺酶功能位点等方面进行预测和分析。结果表明:小菜蛾的9个PGRP中,5个属于S型,4个属于L型;分别定位在细胞质、细胞膜和内质网;有6条PGRP功能位点的分布与蓖麻蚕、棉铃虫、欧洲玉米螟、大蜡螟和印度柞蚕中已知功能的PGRP相似。序列比对的结果表明小菜蛾PGRP之间可能具有不同的功能。
关 键 词: 农业昆虫学;小菜蛾;肽聚糖识别蛋白;生物信息学
Title: Bioinformatics analysis of peptidoglycan recognition proteins in Plutella xylostella
Author: LIANG Hao, HUANG Bin, HOU Youming
Organization: College of Plant Protection, Fujian Agriculture and Forestry University
Abstract: To study the function of peptidoglycan recognition protein (PGRP) in Plutella xylostella L. further, we predicted and analyzed their physical and chemical properties, subcellular location, conserved domains, secondary and tertiary structure of protein and amidase functional sites by bioinformatics tools. The results showed that there were five S-type and four L-type out of the nine PGRP. They located in cytoplasm, cytomembrane, and endoplasmic reticulum, respectively. Distribution of active sites in the 6 PGRP genes of P. xylostella are similar with Samia ricini, Helicoverpa armigera, Ostrinia nubilalis, Galleria mellonella and Antheraea mylitta, respectively. The results of sequence alignment suggest that there are different functions in each PGRP of P. xylostella.
Key words: agricultural entomology; Plutella xylostella; peptidoglycan recognition protein; bioinformatics
发表期数: 2015年11月第21期
引用格式: 梁昊,黄斌,侯有明. 小菜蛾肽聚糖识别蛋白的生物信息学分析[J]. 中国科技论文在线精品论文,2015,8(21):2209-2215.
 
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