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幼虫密度对粘虫免疫能力的影响

发表时间:2015-11-15  浏览量:1376  下载量:573
全部作者: 毛念,王成,孔海龙
作者单位: 扬州大学园艺与植物保护学院
摘 要: 为明确粘虫(Mythimna separata)幼虫密度对其免疫能力的影响作用,在室内条件[温度(23±1)℃,光周期为L:D=16:8,相对湿度70%~75%]下,对不同幼虫密度(1,2,5,10,30头/瓶)饲养下幼虫的血细胞总数、酚氧化酶和溶菌酶活性进行测定。结果表明:幼虫密度对幼虫血细胞总数、酚氧化酶活性影响显著。随着幼虫密度的增加,幼虫血细胞总数显著递增。当幼虫密度达到5头/瓶时,幼虫血细胞总数显著高于1,2头/瓶;30头/瓶的幼虫血细胞总数又显著高于1,2,5,10头/瓶。酚氧化酶活性随幼虫密度的变化趋势与血细胞总数略有不同,当幼虫密度不超过10头/瓶时,幼虫酚氧化酶活性随幼虫密度增加而明显增强,但幼虫密度达到30头/瓶时,幼虫酚氧化酶活性又显著降低。溶菌酶活性在不同密度处理的幼虫之间无显著差异。因此,粘虫幼虫密度主要通过幼虫的血细胞总数和血淋巴酚氧化酶活性影响其免疫能力。
关 键 词: 植物保护学;粘虫;幼虫密度;酚氧化酶活性;溶菌酶活性;血细胞总数
Title: Effects of larval density on immune capacity of oriental armyworm (Mythimna separata)
Author: MAO Nian, WANG Cheng, KONG Hailong
Organization: School of Horticulture and Plant Protection, Yangzhou University
Abstract: To investigate the effects of larval density on immune capacity of the oriental armyworm (Mythimna separata), total hemocyte counts, activities of phenoloxidase and lysozyme of the larvae reared at the densities of 1, 2, 5, 10 and 30 larvae per jar were tested in the laboratory, temperature was (23±1)℃, photoperiod (L:D) was 16:8, relative huminity was 70%-75%. The results showed that total hemocyte counts and phenoloxidase activitiy of larvae were significantly affected by larval density. Total hemocyte counts significantly increased with increasing larval density. Total hemocyte counts of larvae at the density of 5 larvae per jar were significantly higher than that of larvae at densities of 1, 2 larvae per jar. Meanwhile, total hemocyte counts at the density of 30 larvae per jar were significantly higher than that of larvae at densities of 1, 2, 5, 10 larvae per jar. The trend of the activitiy of phenoloxidase with larval density was slightly different from the total hemocyte counts. Phenoloxidase activity in larval haemolymph significantly increased with increasing larval density as the larval density was no more than 10 larvae per jar and it significantly declined at the density of 30 larvae per jar. In addition, larval density had no significant effect on lysozyme activity. These results suggest that the larval density can affect total hemocyte counts and phenoloxidase activitiy in larval haemolymph, and this impact in turn can affect immune capacity of oriental armyworm.
Key words: plant protection; Mythimna separata; larval density; phenoloxidase activitiy; lysozyme activitiy; total hemocyte counts
发表期数: 2015年11月第21期
引用格式: 毛念,王成,孔海龙. 幼虫密度对粘虫免疫能力的影响[J]. 中国科技论文在线精品论文,2015,8(21):2265-2270.
 
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