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不同剂量白藜芦醇对高脂饲喂小鼠非酒精性脂肪肝的影响

发表时间:2015-11-15  浏览量:1549  下载量:511
全部作者: 宋昱,王燕,孙林杰,施用晖,乐国伟
作者单位: 江南大学食品学院食品营养与功能因子研究中心;江南大学食品科学与技术国家重点实验室
摘 要: 探讨不同剂量白藜芦醇(resveratrol,RSV)对高脂膳食诱导的小鼠非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)的影响。将6周龄C57BL/6雄性小鼠随机分为4组:正常日粮组(normal diet,ND)、高脂日粮组(high fat diet,HF)、高脂日粮+0.04%(质量分数)RSV组(HFL)和高脂日粮+0.08%(质量分数)RSV组(HFH)。17周末处死小鼠,显微镜观察肝脏组织切片,测定血脂及肝脏脂类、脂代谢相关基因。与ND组相比,HF组血浆总甘油三酯(total triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low density lipoprotein cholesterol,LDL-C)和谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)水平显著升高;与0.08%RSV相比,0.04%RSV可以显著降低小鼠血浆TG,TC,LDL-C和ALT含量,提高HDL-C水平。RT-PCR结果显示,HF组小鼠脂肪酸合成酶(fatty acid synthetase,Fas)基因、固醇调节元件结合蛋白1c(sterol regulatory element binding protein 1c,SREBP1c)基因、乙酰辅酶a(acetyl-CoA carboxylase,ACC)基因的表达显著上调,腺苷酸活化蛋白激酶(adenosine-5’-monophosphate-activated protein kinase,AMPK)基因、去乙酰化酶(sirtuin type 1,SIRT1)基因、b2肾上腺素受体(b2-adrenergic receptor,b2-AR)基因、肉碱酰基转移酶(carnitine palmitoyltransferase,CPT1)基因的表达显著下调;而HFL小鼠Fas,SREBP1c,ACC基因的表达显著降低,b2-AR,CPT1基因的表达显著升高;0.08%RSV对于b2-AR,CPT1基因表达的影响不显著,而Fas,SREBP1c,ACC基因的表达高于HFL组,并具有显著性差异。因此,以上结果表明对于高脂膳食引起的脂代谢紊乱和NAFLD,0.04%RSV的改善效果优于0.08%的剂量,这可能是由于RSV发挥调节脂类代谢的作用与剂量有着密切关系。
关 键 词: 食品营养学;白藜芦醇;小鼠;脂代谢;非酒精性脂肪肝
Title: Effects of different doses of resveratrol on nonalcoholic fatty liver disease in mice fed with high-fat diet
Author: SONG Yu, WANG Yan, SUN Linjie, SHI Yonghui, LE Guowei
Organization: Center for Food Nutrition & Functional Factors, School of Food Science and Technology, Jiangnan University; State Key Laboratory of Food Science & Technology, Jiangnan University
Abstract: To investigate the impacts of different doses of resveratrol (RSV) on lipid metabolism in high-fat diet (HFD) induced nonalcoholic fatty liver disease (NAFLD) in mice. Six-week-old C57BL/6 male mice were fed with normal diet (normal diet, ND), HFD control (high fat diet, HF), HFD+0.04% (mass percent) RSV (HFL) and HFD+0.08% (mass percent) RSV(HFH). Mice were sacrificed after 17 weeks. Liver histological analysis was performed by microscopy. Plasma and hepatic lipid profile and lipid metabolism related genes were measured. Compared with the ND group, the levels of total triglyceride (TG), total-cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and alanine transaminase (ALT), aspartate aminotransferase (AST) were significantly elevated in the plasma of HF mice. Supplemented with 0.04%RSV was more effective than 0.08%RSV in reducing the levels of TG, TC and ALT, and improving the level of HDL-C. RT-PCR results showed that the gene expressions of fatty acid synthetase (Fas), sterol regulatory element binding protein 1c (SREBP1c) and acetyl-CoA carboxylase (ACC) were significantly up-regulated, while adenosine-5’-monophosphate-activated protein kinase (AMPK), sirtuin type 1 (SIRT1), carnitine palmitoyltransferase (CPT1) and b2-adrenergic receptor (β2-AR) were greatly down-regulated in HF mice. HFL mice had low expressions of Fas, SREBP1c and ACC gene, and high expressions of b2-AR and CPT1 gene. Treated with 0.08% RSV had no remarkable effect on the expression of b2-AR and CPT1 gene, while the expressions of Fas, SREBP1c and ACC gene were higher compared with HFL group. Thus, these results indicated that for a HFD caused by lipid metabolism disorders and NAFLD, 0.04%RSV was more effect than 0.08%RSV, which may be due to the effects of RSV on lipid metabolism were closely related to the dosage of RSV.
Key words: food nutriology; resveratrol; mice; lipid metabolism; nonalcoholic fatty liver disease
发表期数: 2015年11月第21期
引用格式: 宋昱,王燕,孙林杰,等. 不同剂量白藜芦醇对高脂饲喂小鼠非酒精性脂肪肝的影响[J]. 中国科技论文在线精品论文,2015,8(21):2284-2291.
 
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