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新型补钙试剂的制备及其吸收特性研究

发表时间:2015-11-15  浏览量:1497  下载量:502
全部作者: 李美良,周凯,赵广华
作者单位: 中国农业大学食品科学与营养工程学院
摘 要: 为补充和强化膳食中匮乏的钙元素,研究利用植物铁蛋白特有的纳米空腔结构,通过与钙离子共孵育,制备出一种溶解性好、可食用的新型钙 铁蛋白复合物作为新型的补钙试剂。铁蛋白的空腔结构作为一个良好的生物载体,替代了有机、无机载体,实现了钙离子在体内的运输,且蛋白质外壳可保护钙离子免于与膳食中的单宁酸(tannic acid,TA)、草酸(oxalic acid,OA)和其他二价金属离子等接触,从而提高了钙离子的生物利用率。研究发现,钙 铁蛋白复合物在Caco-2细胞中的吸收通过TfR1受体介导完成,与游离钙离子吸收的受体(DMT1)不同,说明钙 铁蛋白复合物中的钙离子在Caco-2细胞中的吸收与一般的补钙试剂不同,其通过Caco-2细胞的内化实现。因此,钙 铁蛋白复合物可以作为一种有效的新型补钙试剂。
关 键 词: 食品生物化学;钙 铁蛋白复合物;Caco-2细胞;钙吸收;TfR1;补钙试剂
Title: Preparation of a novel calcium supplement and its absorption characteristic
Author: LI Meiliang, ZHOU Kai, ZHAO Guanghua
Organization: College of Food Science & Nutritional Engineering, China Agricutural University
Abstract: In this work, we prepared a novel class of calcium supplement of soluble and edible Ca-ferritin complexes through incubating with calcium ions to strengthen the calcium content in food. As an alternative to other organic and/or inorganic carriers, protein nanocages were found to provide a unique vehicle of biological origin for the intracellular delivery of calcium ions for supplementation. Such encapsulation can protect calcium ions within protein cages against dietary factors such as tannic acid (TA), oxalic acid (OA), and other divalent metal ions in foodstuffs. We demonstrated that the Ca-ferritin complexes can be absorbed by Caco-2 cells through a process where a TfR1 receptor is involved, which is different from the receptor (DMT1) of free calcium ions, indicating that the calcium ions encapsulated in supramolecular protein cages can be internalized by the Caco-2 cells through a different pathway from its free analogs for calcium supplementation. In consequence, the Ca-ferritin complexes can be used as an novel calcium supplement which is efficient.
Key words: food biochemistry; Ca-ferritin complexes; Caco-2 cells; calcium absorption; TfR1; calcium supplement
发表期数: 2015年11月第21期
引用格式: 李美良,周凯,赵广华. 新型补钙试剂的制备及其吸收特性研究[J]. 中国科技论文在线精品论文,2015,8(21):2292-2302.
 
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