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能源小球藻异养培养条件优化研究

发表时间:2015-12-31  浏览量:1795  下载量:631
全部作者: 黄佳,张文君,刘珺,李彦鹏
作者单位: 长安大学环境科学与工程学院;长安大学旱区地下水文与生态效应教育部重点实验室
摘 要: 对影响小球藻生长的4个主要因素即葡萄糖,KNO3,KH2PO4及MgSO4进行单因素实验,探讨不同添加量对生物量的影响。并通过正交实验确定各因素对小球藻影响程度的大小,得到了异养培养的最佳优化条件:在BG11培养基的基础上,葡萄糖浓度为20 g/L, C∶N(质量比)为3:1,KH2PO4浓度为 0.05 g/L,MgSO4浓度为 0.15 g/L时,小球藻的最大生物量可达到4.45 g/L. 该研究为小球藻的异养培养提供了依据。
关 键 词: 环境生物学;小球藻;异养;优化
Title: Study on the heterotrophic cultivation optimization of Chlorella sp.
Author: HUANG Jia, ZHANG Wenjun, LIU Jun, LI Yanpeng
Organization: School of Environmental Science and Engineering, Chang’an University; Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang’an University
Abstract: To examine the effect of glucose, KNO3, KH2PO4 and MgSO4 on the growth of Chlorella sp., single factor and orthogonal experiments were employed to determine the optimum condition of heterotrophic cultivation. Results showed that on the basis of BG11 culture medium with the condition of glucose 20 g/L, C:N 3:1 (mass ratio), KH2PO4 0.05 g/L, MgSO4 0.15 g/L, Chlorella sp. obtained the maximum biomass 4.45g/L .The research provided basis for heterotrophic cultivation of Chlorella sp.
Key words: environmental biology; Chlorella sp.; heterotrophic; optimization
发表期数: 2015年12月第24期
引用格式: 黄佳,张文君,刘珺,等. 能源小球藻异养培养条件优化研究[J]. 中国科技论文在线精品论文,2015,8(24):2530-2538.
 
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