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活性污泥和生物膜中紧密结合型胞外聚合物提取方法研究

发表时间:2015-12-31  浏览量:1896  下载量:579
全部作者: 曾妮,陈猷鹏,刘嘉靖,王一璐,赵尚q
作者单位: 重庆大学城市建设与环境工程学院
摘 要: 用4种物理方法[阳离子交换树脂(cationic exchange resin,CER)法、热提取法、蒸汽法和超声法]提取来自基于亚硝酸盐的全自养生物脱氮(completely autotrophic nitrogen removal over nitrite,CANON)工艺活性污泥和生物膜以及厌氧-缺氧-好氧(anaerobic-anoxic-oxic,A/A/O)工艺中反硝化污泥中的紧密结合型胞外聚合物(tightly bound extracellular polymeric substances,TB-EPS)。用拉曼光谱表征TB-EPS的官能团生化信息,通过测定TB-EPS中蛋白质、多糖、腐殖酸及DNA的含量以研究TB-EPS的组成,并比较4种提取方法优劣。结果表明,不同提取方法和微生物聚集方式得到的TB-EPS具有不同的拉曼光谱特征。蛋白质和多糖是CANON系统中TB-EPS的主要成分,而反硝化活性污泥的TB-EPS主要成分为蛋白质和腐殖酸。TB-EPS提取产量强烈依赖于采用的提取方法和微生物的集聚方式,CANON系统中悬浮污泥和生物膜最优提取TB-EPS方法为CER法,A/A/O系统反硝化活性污泥较优提取TB-EPS方法为热提取法与蒸汽法。
关 键 词: 环境生物学;紧密结合型胞外聚合物;活性污泥;生物膜;拉曼光谱法;提取方法
Title: Study of the extraction methods of tightly bound extracellular polymeric substances extracted from activated sludge and biofilm
Author: ZENG Ni, CHEN Youpeng, LIU Jiajing, WANG Yilu, ZHAO Shangyu
Organization: College of Urban Construction and Environmental Engineering, Chongqing University
Abstract: In this study, the tightly bound extracellular polymeric substances (TB-EPS) of the activated sludge and biofilm from a completely autotrophic nitrogen removal over nitrite (CANON) system and the activated sludge from a denitrification process of a anaerobic-anoxic-oxic (A/A/O) system were extracted by four physical extraction methods [cationic exchange resin (CER), heating, steam and ultrasound]. The functional groups of the TB-EPS were analysed by Raman spectrometry. Constitutes of the TB-EPS were investigated by the determination of its protein, polysaccharide, humic acid and DNA contents to analyze the advantadges and disadvantages of the four extraction methods. The results showed that the Raman spectra of TB-EPS differed with the different extraction methods. Proteins and polysaccharides were found to be the main components in the TB-EPS from the CANON system, however, the main components in the TB-EPS of denitrification activated sludge were protein and humic acid. The yields of the TB-EPS extracts were strongly dependent on the extraction methods adopted and the attached type of microbial aggregates. CER was the most effective method in extracting TB-EPS from both sludge and biofilm samples of CANON system, whereas the better extraction methods for the TB-EPS from denitrification activated sludge samples were heating and steam.
Key words: environmental biology; tightly bound extracellular polymeric substances; activated sludge; biofilm; Raman spectrymetry; extraction methods
发表期数: 2015年12月第24期
引用格式: 曾妮,陈猷鹏,刘嘉靖,等. 活性污泥和生物膜中紧密结合型胞外聚合物提取方法研究[J]. 中国科技论文在线精品论文,2015,8(24):2539-2545.
 
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