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拟南芥钙调素类似蛋白CML20参与植物抗干旱研究

发表时间:2016-02-15  浏览量:2426  下载量:819
全部作者: 吴晓萌,李春龙,钱景美,郑昱,张伟
作者单位: 山东大学生命科学学院,植物细胞工程与种质创新教育部重点实验室;山东大学生命科学学院
摘 要: 干旱是限制植物生长发育和作物产量最主要的逆境环境之一,因此,筛选参与植物干旱胁迫响应的功能基因是发展节水、耐逆现代化农业的基础。本研究发现,拟南芥类钙调素蛋白CML20参与介导了植物的干旱胁迫响应,其功能缺失突变体比野生型更耐干旱。为明确CML20参与植物干旱胁迫响应的作用机理,对其编码基因表达模式、蛋白性质及参与脱落酸(abscisic acid,ABA)调控气孔运动的生理功能进行初步研究与分析。结果表明,CML20在植物多个组织部位都有表达,其中在保卫细胞表达量较高;其表达蛋白定位于细胞质,而且该蛋白具有钙离子结合活性。生理性状实验表明,缺失表达突变体cml20对ABA调控气孔运动过程过敏感,同时抗干旱能力增强。综合分析,CML20可能是响应胞质内钙离子的下游负调控因子,参与介导ABA调控气孔运动过程,进而影响植物抗干旱能力。
关 键 词: 细胞生物学;气孔运动;保卫细胞;脱落酸;干旱胁迫
Title: Plant drought response evokes CML20, a calmodulin-like protein of Arabidopsis thaliana
Author: WU Xiaomeng, LI Chunlong, QIAN Jingmei, ZHENG Yu, ZHANG Wei
Organization: Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University; School of Life Science, Shandong University
Abstract: Drought is one of the most serious abiotic stresses in restraining of plant growth and development as well as crop yields. To screen the plant drought responsive genes and elucidate their functional molecular mechanism will be essentially needed for development of stress-tolerant, water-efficient agriculture. In the current study, the CML20, a member of Arabidopsis calmodulin-like proteins was found to be negatively involved in abscisic acid (ABA) guard cell signaling and plant drought response. CML20 was ubiquitously expressed in a serial of tissues, including roots, leaves and flowers, and especially with a high expression in guard cells. The CML20 was Ca2+-binding protein and subcellularly localized in cytosol. Physiological experiments showed that the cml20, the loss-of-function mutant of CML20, was hypersensitive to ABA regulated stomatal movements than wild type and thus to diminish water loss to enhance the plant drought tolerance. All presented data demonstrated that CML20 may function as a negative regulator in ABA guard cell signaling and plant drought response.
Key words: cell biology; stomatal movements; guard cell; abscisic acid; drought stress
发表期数: 2016年2月第3期
引用格式: 吴晓萌,李春龙,钱景美,等. 拟南芥钙调素类似蛋白CML20参与植物抗干旱研究[J]. 中国科技论文在线精品论文,2016,9(3):205-212.
 
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