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2,4-二氯苯氧乙酸与小牛胸腺DNA的沟槽结合作用

发表时间:2016-02-15  浏览量:1891  下载量:681
全部作者: 夏开心,张国文
作者单位: 南昌大学食品科学与技术国家重点实验室
摘 要: 在生理酸度(pH=7.4)条件下,采用多种光谱学技术包括,紫外-可见吸收光谱法(ultraviolet-visible absorption,UV-vis)、荧光光谱法、圆二色谱法(circular dichroism,CD)及红外光谱法(Fourier transform infrared apectroscopy,FT-IR)结合分子模拟的方法,研究2,4-二氯苯氧乙酸(简记为2,4-D)与小牛胸腺DNA(calf thymus DNA,ctDNA)的体外结合性质,并分析其相互作用机制。2,4-D对ctDNA的熔点和黏度影响不明显,以及与溴化乙锭(ethidium bromide,EB)、吖啶橙(acridine orange,AO)探针竞争实验结果显示,2,4-D主要通过沟槽方式与ctDNA相互作用。FT-IR和CD分析表明,2,4-D优先结合在ctDNA的A、T碱基上,并在一定程度上对ctDNA的碱基堆积和双螺旋结构产生干扰,使ctDNA的构象由B型向A型转变,分子模拟结果证实了实验结果并直观展示了2,4-D与ctDNA的结合模式及空间结合姿态。凝胶电泳实验表明,2,4-D对pUC18质粒DNA未产生明显损伤。
关 键 词: 食品添加剂技术;2,4-二氯苯氧乙酸;小牛胸腺DNA;结构模式;光谱法;分子模拟
Title: Groove binding interaction between 2, 4-dichlorophenoxyacetic acid and calf thymus DNA
Author: XIA Kaixin, ZHANG Guowen
Organization: State Key Laboratory of Food Science and Technology, Nanchang University
Abstract: The interaction between 2,4-dichlorophenoxyacetic acid (2,4-D) and calf thymus DNA (ctDNA) in vitro was investigated by multispectroscopic methods containing ultraviolet-visible absorption (UV-vis), fluorescence, circular dichroism (CD) and Fourier transform infrared apectroscopy (FT-IR) with molecular modeling under simulate physiological conditions (pH=7.4). The 2,4-D caused little change in melting temperature and viscosity of ctDNA, and the competition experiments of ethidium bromide (EB) and acridine orange (AO) confirmed that the groove binding occurred between 2,4-D and ctDNA. Analysis of the FT-IR and CD spectra indicated that 2,4-D preferentially bound to adenine and thymine bases, and perturbed the base stacking and double helix of ctDNA, leading to a transformation from the B-form of ctDNA to A-form, and the molecular docking further verified the results of experiments and intuitively showed the binding pose of 2,4-D with ctDNA. Additionally, the gel electrophoresis results indicated that 2,4-D did not induce any obvious cleavage of supercoiled pUC18 plasmid DNA.
Key words: food additives technology; 2,4-dichlorophenoxyacetic acid; calf thymus DNA; binding mode; spectroscopy; molecular modeling
发表期数: 2016年2月第3期
引用格式: 夏开心,张国文. 2,4-二氯苯氧乙酸与小牛胸腺DNA的沟槽结合作用[J]. 中国科技论文在线精品论文,2016,9(3):284-293.
 
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