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Kindlin-2通过抑制microRNA-200家族调节肿瘤细胞的侵袭转移

发表时间:2016-03-15  浏览量:1436  下载量:621
全部作者: 祁丽花,于宇,张宏权
作者单位: 北京大学基础医学院
摘 要: 为探索整合素相互作用蛋白Kindlin-2调控肿瘤细胞侵袭转移的分子机制,研究进行了大规模的microRNA (miRNA)表达谱分析,发现Kindlin-2过表达可以显著下调miRNA-200家族(miR-200a/200b/200c/141)的表达水平。同时,通过mRNA表达谱的分析发现,Kindlin-2可以调控许多与肿瘤侵袭转移相关的信号通路的基因表达,包括Wnt信号通路、 Hedgehog信号通路以及上皮细胞间质转化(epithelial-mesenchymal transition,EMT)过程。特别地,Kindlin-2还可以显著调控miRNA-200的靶基因ZEB1的表达。更为重要的是,研究发现miRNA-200的过表达可以部分抑制Kindlin-2对肿瘤细胞侵袭的促进作用。因此,本研究将通过表达谱的分析揭示Kindlin-2调节肿瘤侵袭的分子机制。
关 键 词: 医学细胞生物学;Kindlin-2;microRNA-200;细胞侵袭
Title: Regulation of Kindlin-2 on tumor cell invasion and metastasis via inhibiting microRNA-200 family
Author: QI Lihua, YU Yu, ZHANG Hongquan
Organization: School of Basic Medical Sciences, Peking University
Abstract: To investigate the molecular mechanism of integrin-interacting protein Kindlin-2 regulating tumor cell invasion and metastasis, by analysis of microRNA (miRNA) microarrays, we find that over-expression of Kindlin-2 obviously down-regulates the levels of miRNA-200 family (miR-200a/200b/200c/141). Meanwhile, results of mRNA microarrays showed that Kindlin-2 could regulate some gene expression of tumor related signaling pathway, including Wnt signaling genes, Hedgehog signaling genes and epithelial-mesenchymal transition (EMT) related genes. Particularly, Kindlin-2 promotes the expression of ZEB1, a target gene of miRNA-200. More importantly, we find that over-expression of miRNA-200 can partly rescue the promotion of Kindlin-2 on tumor cell invasion. Thus, this research will reveal some new molecular mechanism of regulation of Kindlin-2 on tumor cell invasion by analysis of gene microarray.
Key words: medical cell biology; Kindlin-2; microRNA-200; cell invasion
发表期数: 2016年3月第5期
引用格式: 祁丽花,于宇,张宏权. Kindlin-2通过抑制microRNA-200家族调节肿瘤细胞的侵袭转移[J]. 中国科技论文在线精品论文,2016,9(5):456-463.
 
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