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白头翁皂苷A对非小细胞肺癌放射敏感性影响的初步研究

发表时间:2016-03-15  浏览量:1330  下载量:477
全部作者: 彭超军,焦旸
作者单位: 苏州大学医学部放射医学与防护学院
摘 要: 目的:初步研究白头翁皂苷A(Pulsatilla saponin A,PsA)作为肺癌放射治疗增敏剂的可行性。方法:利用噻唑蓝(MTT)细胞存活实验、克隆形成实验、流式细胞术(flow cytometry,FCM)分析、免疫荧光染色和单细胞凝胶电泳等, 对受到X射线照射的实验组和对照组H1975细胞的存活率、细胞周期分布、DNA损伤情况进行检测。结果:与人类正常支气管上皮细胞相比,PsA对人类非小细胞肺癌细胞H1975具有显著的增殖抑制作用。PsA显著抑制受照射后H1975细胞的克隆形成能力;PsA引起H1975细胞发生G2/M期阻滞,并联合X射线进一步增加细胞的G2/M期阻滞;PsA可进一步增加X射线引起的H1975细胞DNA双链断裂。结论:PsA可增加非小细胞肺癌细胞H1975对X射线的敏感性,其机制可能与其影响肺癌细胞受照射后的DNA损伤有关;PsA具备作为放射治疗增敏剂的潜能。
关 键 词: 放射医学;放射敏感性;白头翁皂苷A;非小细胞肺癌;电离辐射;DNA双链断裂
Title: Preliminary study of effects of Pulsatilla saponin A on non-small cell lung cancer radiosensitivity
Author: PENG Chaojun, JIAO Yang
Organization: School of Radiation Medicine and Protection, Medical College of Soochow University
Abstract: Objective: To investigate the potential application of Pulsatilla saponin A (PsA) as a radiosensitizer of human non-small cell lung cancer. Methods: MTT viability assay, clonogenic assay, flow cytometry (FCM) analysis, immunoflourescence staining, and single cell gel eletrophoresis assay were utilized to determine the viability, cell cycle distribution, and DNA damage of PsA treated H1975 cells and control cells, with or without X-ray exposure. Results: Compared with the effects on normal human bronchial epithelial cell line, PsA obviously suppressed the cell viability of non-small cell lung cancer cell line H1975. PsA significantly impaired the clonogenic ability of H1975 cells after exposed to X-ray irradiation, caused and enhanced the G2/M phase cell population of H1975 cells after irradiation. In addition, PsA could increase the X-ray induced DNA double strand breaks of H1975 cells. Conclusion: PsA could sensitize H1975 cells to X-ray irradiation, probably via its regulation on irradiation induced DNA damage, which indicates the possibility of PsA as a promising radiosensitizer for cancer radiotherapy.
Key words: radioactive medicine; radiosensitivity; Pulsatilla saponin A; non-small cell lung cancer; ionizing irradiation; DNA double strand break
发表期数: 2016年3月第5期
引用格式: 彭超军,焦旸. 白头翁皂苷A对非小细胞肺癌放射敏感性影响的初步研究[J]. 中国科技论文在线精品论文,2016,9(5):464-469.
 
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