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茯苓多糖对免疫抑制小鼠GALT淋巴细胞活化和迁移的影响

发表时间:2016-03-15  浏览量:1481  下载量:618
全部作者: 邓向亮,黄容容,罗霞,周联
作者单位: 广州中医药大学中药学院;无限极(中国)有限公司研发中心
摘 要: 目的:考察茯苓多糖对环磷酰胺诱导免疫抑制小鼠肠相关淋巴组织(gut associated lymphoid tissue,GALT)淋巴细胞活化和迁移的影响。方法:将60只BALB/c小鼠随机均分为对照组、模型组和茯苓多糖高、中、低剂量组。茯苓多糖高、中、低剂量组小鼠分别按400,200,100 mg/kg剂量灌服茯苓多糖溶液,对照组和模型组小鼠灌服等量生理盐水,每天给药1次,连续10 d. 给药第6天,模型组和茯苓多糖各剂量组腹腔注射环磷酰胺(100 mg/kg),对照组腹腔注射等体积生理盐水。末次给药第2天,取肠系膜淋巴结(mesenteric lymph node,MLN)、派氏结(Peyer’s patches,PPs)制备淋巴细胞悬液,采用流式细胞术检测淋巴细胞表面CD69、CCR7分子表达水平;同时取小肠制作苏木精-伊红(hematoxylin-eosin,HE)染色切片,光镜下观察PPs形态。结果:与对照组比较,模型组MLN中T、B细胞和PPs中T细胞表达CD69分子水平降低,PPs中B细胞表达CD69分子水平升高(P<0.05);而与模型组比较,茯苓多糖各剂量组MLN和PPs中B细胞表达CD69分子水平显著恢复(P<0.05)。茯苓多糖各剂量组MLN淋巴细胞表达CCR7分子水平均高于模型组,中、低剂量组与模型组比较,差异具有统计学意义(P<0.05)。HE染色切片结果显示,模型组PPs面积显著小于正常对照组,而茯苓多糖组有所恢复。结论:茯苓多糖具有调节小鼠GALT B细胞活化水平、促进淋巴细胞迁移的作用。
关 键 词: 中西医结合医学;茯苓多糖;肠相关淋巴组织;CCR7;CD69
Title: Effect of pachymaran on activation and migration of lymphocytes in GALT of immunosuppression mice
Author: DENG Xiangliang, HUANG Rongrong, LUO Xia, ZHOU Lian
Organization: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine; Research & Development Centre, Infinitus (China) Co. Ltd.
Abstract: Objective: To investigate the effect of pachymaran on lymphocytes activation and migration in gut associated lymphoid tissue (GALT) of immunosuppression mice induced by cyclophosphamide. Methods: 60 BALB/c mice were randomly divided into control, model and pachymaran high, medium and low dose groups. Mice in pachymaran groups were ig administered with pachymaran 400, 200, 100 mg/kg, once per day for 10 d, while those in control group and model group were ig administered with the same dosages of saline solutions. On the sixth day, mice in model group and pachymaran groups were ip administered with cyclophosphamide (100 mg/kg), and those in control group were ip administered with the same volume of saline solutions. The second day after the last administration, lymphocytes suspension from mesenteric lymph nodes (MLN) and Peyer’s patches (PPs) was prepared. The expression levels of CD69 and CCR7 molecules on lymphocytes surface were detected by flow cytometry. The morphologies of PPs were observed under microscope after hematoxylin-eosin (HE) staining. Results: Compared with control group, CD69 molecule expression levels were decreased on T and B cells of MLN and on T cells of PPs in model group, while that was increased on B cells of PPs (P<0.05). Compared with model group, CD69 molecule expression levels were recovery on B cells of MLN and PPs in pachymaran groups (P<0.05). CCR7 molecule expression levels in pachymaran groups were higher than those in model group, and it was significant different in pachymaran medium and low dose group compared with model group (P<0.05). HE staining results showed that the area of PPs was smaller in model group than that in control group, but it was significant recovered in pachymaran groups. Conclusion: Pachymaran can regulate the activation level of B cells and promote the migration of lymphocytes in GALT.
Key words: Chinese and Western integrative medicine; pachymaran; gut associated lymphoid tissue; CCR7; CD69
发表期数: 2016年3月第5期
引用格式: 邓向亮,黄容容,罗霞,等. 茯苓多糖对免疫抑制小鼠GALT淋巴细胞活化和迁移的影响[J]. 中国科技论文在线精品论文,2016,9(5):484-490.
 
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