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广藿香青枯病菌遗传多样性分析

发表时间:2016-03-15  浏览量:1302  下载量:747
全部作者: 邓志成,贺红,金华,张宇瑶,李颛
作者单位: 广州中医药大学中药学院
摘 要: 目的:研究广藿香青枯病菌的遗传多样性,探讨菌株间的遗传分化情况。方法:观察广藿香青枯病菌在2, 3, 5-氯化三苯基四氮唑(TTC)培养基上的菌落形态;利用16S核糖体DNA(ribosomal DNA,rDNA)扩增片段限制性内切酶分析(amplified 16S rDNA restriction analysis,16S-ARDRA)技术及BOX插入因子PCR技术(BOX-A1R-based repetitive extragenic palindromic-PCR,BOX-PCR),对11个供试菌株进行遗传多样性分析。结果:供试菌株在TTC培养基上主要呈现4种菌落形态,16S-ARDRA聚类分析共获得4个16S-ARDRA型,其中类型A为主要类型,共含有8个菌株;BOX-PCR聚类分析结果显示,所有菌株在48%相似性水平上与16S-ARDRA多态性分析结果相似,而在84%的相似水平上,类型A被进一步分为3个亚群。结论:广藿香青枯病菌具有一定的遗传多样性;在多样性测定方面,BOX-PCR技术具有更高的分辨率。
关 键 词: 药用植物学;广藿香;青枯病菌;遗传多样性;16S-ARDRA;BOX-PCR
Title: Genetic diversity of Ralstonia solanacearum strains isolated from Pogostemon cablin (Blanco) Benth.
Author: DENG Zhicheng, HE Hong, JIN Hua, ZHANG Yuyao, LI Zhuan
Organization: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine
Abstract: Objective: To study on the genetic diversity and differentiation of Ralstonia solanacearum strains isolated from Pogostemon cablin (Blanco) Benth. Methods: Differences in the colonial morphology of R. solanacearum strains were observed on triphenyl tetrazolium chloride (TTC) medium. Further, genetic diversity among the 11 strains was analyzed by amplified 16S ribosomal DNA restriction analysis (16S-ARDRA) and BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR). Results: When observed on TTC medium, the strains could be divided into four groups according to their colonial morphology. Based on 16S-ARDRA patterns, the strains could be grouped into four 16S-ARDRA types. Type A was the main type, which contained 8 strains. At the 48% similarity level, similar results were obtained by using BOX-PCR fingerprint technique. While at 84% similarity level, type A could be further divided into three sub-groups by BOX-PCR fingerprint. Conclusion: A significant level of genetic diversity exists among R. solanacearum strains isolated from P. cablin (Blanco) Benth. Among the strains, BOX-PCR analysis shows higher discriminatory power than 16S-ARDRA technique in the determination of genetic diversity.
Key words: medicinal botany; Pogostemon cablin (Blanco) Benth.; Ralstonia solanacearum; genetic diversity; 16S-ARDRA; BOX-PCR
发表期数: 2016年3月第5期
引用格式: 邓志成,贺红,金华,等. 广藿香青枯病菌遗传多样性分析[J]. 中国科技论文在线精品论文,2016,9(5):498-504.
 
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