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一种改良的恒河猴和食蟹猴促排卵后取卵术

发表时间:2016-05-15  浏览量:1723  下载量:636
全部作者: 高家红,黄璋琼,江勤芳,曾桥,吴正存
作者单位: 中国医学科学院北京协和医学院医学生物学研究所药物安全性评价研究中心;同济大学附属同济医院转化医学中心
摘 要: 目的:探索一种经改良、微创、高效的恒河猴和食蟹猴促排卵后的手术取卵方法。方法:采用B超对促排卵后的食蟹猴和恒河猴卵巢进行定位及尺寸测量,然后采用自制的导尿管导尿以解除膀胱对卵巢的遮挡,在下腹部中央或两侧(靠近卵巢上方)行1.5~2.5 cm的小切口,暴露卵巢,然后用5 mL注射器配置23 Gauge针头负压抽吸卵泡。结果:共对11只恒河猴和13只食蟹猴行改良取卵术,恒河猴卵巢平均大小为2.2 cm×2.4 cm,食蟹猴卵巢平均大小为2.1 cm×2.4 cm,恒河猴手术切口平均大小为(2.3±0.2)cm,食蟹猴手术切口平均大小为(2.2±0.1)cm,手术总体平均时间为(44±10)min,恒河猴平均获卵数为(26.5±15.8)个,食蟹猴平均获卵数为(25.1±16.3)个。结论:在B超下确认卵泡位置和大小、导尿后在下腹部中央或两侧行小切口的取卵术是一种可行、低创伤、高效的取卵方式。
关 键 词: 动物学;恒河猴;食蟹猴;促排卵;B超定位;导尿
Title: An improved oocyte retrieval after superovulation on rhesus and cynomolgus monkeys
Author: GAO Jiahong, HUANG Zhangqiong, JIANG Qinfang, ZENG Qiao, WU Zhengcun
Organization: Center for Drug Safety Evaluation Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College; Center for Translational Medicine, Tongji Hospital Affiliated to Tongji University
Abstract: Objective: To explore an improved, high efficient and mild hurt operation technique for oocyte retrieval in rhesus and cynomolgus monkeys. Methods: To locate and measure ovum with B-type ultrasonography after superovulation in rhesus and cynomolgus monkeys. We catheterized with self-made catheter to relieve occulusion of bladder, and then we operated an 1.5-2.5 cm incision in the center of hypogastrium or two incisions of two hypogastrium sides, respectively. The ovary was exposured, and then aspirated ovarian follicles under negative pressure with a 5 mL syringe which attached 23 Gauge needle. Results: 11 rhesus monkeys and 13 cynomolgus monkeys had been undergone this improved operation. The average size of ovum was 2.2 cm×2.4 cm in rhesus monkeys and 2.1 cm×2.4 cm in cynomolgus monkeys. The average size of incisions was (2.3±0.2) cm in rhesus monkeys and (2.2±0.1) cm in cynomolgus monkeys. The total average operation time was (44±10) min. The average number of oocyte retrieval was 26.5±15.8 in rhesus monkeys and 25.1±16.3 in cynomolgus monkeys. Conclusions: It is a feasible, high efficient and low hurt way that we cut a small incision for oocyte retrieval after catheterization through locating and measuring ovum with B-type ultrasonography.
Key words: zoology; rhesus monkey; cynomolgus monkey; superovalution; B-type ultrasonography; urethral catheterization
发表期数: 2016年5月第9期
引用格式: 高家红,黄璋琼,江勤芳,等. 一种改良的恒河猴和食蟹猴促排卵后取卵术[J]. 中国科技论文在线精品论文,2016,9(9):881-886.
 
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