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二穗短柄草b型Farinin基因的克隆与进化分析

发表时间:2016-05-15  浏览量:1699  下载量:639
全部作者: SUBBURAJ Saminathan,朱耿锐,李晓辉,胡英考,晏月明
作者单位: 首都师范大学遗传与生物工程重点实验室
摘 要: Farinin是小麦和山羊草属等物种中最古老的储藏蛋白家族成员之一,对面团特性具有重要影响。本研究对二穗短柄草(Brachypodium distachyon L.)种子Farinin蛋白基因进行了分子遗传学分析。采用等位基因特异PCR(allele-specific PCR,AS-PCR)方法从14个不同二穗短柄草材料中分离、克隆了51个b型Farinin基因。序列比对显示,克隆的基因与先前报道的小麦和山羊草属物种中的燕麦类似蛋白基因具有高度的相似性。氨基酸序列表明,小麦中b型Farinin分为b1、b2、b3和b4四种类型,而在二穗短柄草中只含有b3和b4两种类型。通过系统进化分析,发现短柄草Farinin与普通小麦中成员的关系更近,也进一步证实了Farinin家族存在Group 1(b1、b2和b3)和Group 2(b4)两大亚家族,而b4型的Farinin是这个家族中较为古老的成员。
关 键 词: 植物学;二穗短柄草;Farinin;分子克隆;进化
Title: Molecular characterization and evolutionary analysis of b-type Farinin genes in Brachypodium distachyon L.
Author: SUBBURAJ Saminathan, ZHU Gengrui, LI Xiaohui, HU Yingkao, YAN Yueming
Organization: Key Laboratory of Genetics and Biotechnology, Capital Normal University
Abstract: Farinin is one of the oldest members of the gluten family in wheat and Aegilops species, and related to dough properties. Here, we performed the detailed molecular genetic study on Farinin genes in Brachypodium distachyon L. A total of 51 b-type Farinin genes were cloned and characterized from 14 different B. distachyon L. accessions by allele-specific PCR (AS-PCR). All genes were highly similar to those previously reported from wheat and Aegilops species. The identification of deduced amino acid sequences showed that b-type Farinins across Triticeae genomes could be classified as b1-, b2-, b3-, and b4-type Farinin, while B. distachyon L. had only b3- and b4-type Farinin. Phylogenetic analysis suggested that Brachypodium Farinin may have closer relationships with common wheat and further confirmed four different types of b-type Farinin present in Triticeae and Brachypodium genomes, corresponding to b1, b2, b3 (Group 1), and b4 (Group 2). The b4-type could be considered older in the Farinin family.
Key words: botany; Brachypodium distachyon L.; Farinin; molecular cloning; evolution
发表期数: 2016年5月第9期
引用格式: SUBBURAJ Saminathan,朱耿锐,李晓辉,等. 二穗短柄草b型Farinin基因的克隆与进化分析[J]. 中国科技论文在线精品论文,2016,9(9):887-892.
 
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