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椭圆叶花锚种子休眠机制及其破除方法的研究

发表时间:2016-05-15  浏览量:1643  下载量:518
全部作者: 陈荣健,封 烁,虞红强,王君武,袁建立
作者单位: 兰州大学生命科学学院草地与农业生态系统国家重点实验室
摘 要: 椭圆叶花锚种子萌发率低,休眠程度深。通过测定椭圆叶花锚种子生活力、吸水率以及不同处理下种子的萌发率,来揭示其休眠机制,并探索打破休眠的最佳方案。结果表明:1)椭圆叶花锚种子活力高,氯化三苯基四氮唑(triphenyl tetrazolium chloride,TTC)法测得其生活力可达到(85.33±2.49)%. 2)破皮种子和完整种子的吸水率在14 h之前有差异,但最终的吸水率无差异,分别达到了(92.64±0.88)%和(92.95±0.82)%,说明种皮对种子吸水没有阻碍作用;NaOH处理显著地提高了椭圆叶花锚种子萌发率(P<0.05),说明种皮机械阻碍是引起休眠的因素之一。经NaOH和赤霉素综合处理的种子萌发率得到了极显著地提高(P<0.01),表明种内抑制物是影响休眠的另一因素。因此,认为椭圆叶花锚种子的休眠是种皮和种内抑制物引起的综合休眠。3)不同方法处理的种子的最终萌发率都有不同程度的提高,其中蒸馏水浸种2 d可以使种皮膨胀,不断吸收萌发所需要的水分;用30% NaOH处理80 min,再加以200 g/g赤霉素处理,之后在25℃下萌发,效果最好,萌发率达到(74.80±1.71)%.
关 键 词: 生态学;椭圆叶花锚;休眠机制;萌发率;休眠破除
Title: Study on seed dormancy mechanism and breaking technique of Halenia elliptica
Author: CHEN Rongjian, FENG Shuo, YU Hongqiang, WANG Junwu, YUAN Jianli
Organization: State Key Laboratory of Grassland and Agro-Ecosystems, School of Life Science, Lanzhou University
Abstract: The germination rate of Halenia elliptica was low, and the degree of dormance was deep. The seed dormancy mechanism was revealed, and the best solution of breaking seed dormancy was explored by determining seed viability, water absorption and germination rate of seeds under different treatments. The results showed that: 1) The activity of H. elliptica seed was high, the life force of the seed can be reached (85.33+2.49)% by triphenyl tetrazolium chloride (TTC). 2) The water absorption rates of broken and intact seeds were different in the early 14 h, but the ultimate water absorption rates were no difference, which reached (92.64±0.88)% and (92.95±0.82)% respectively, suggestting that seed coat had no effect on seed water absorption. The germination rate of H. elliptica seed was significantly increased after NaOH treatment (P<0.05), which suggested that the seed capsule barrier was one of factors of the seed dormance. The seed germination rate was significantly improved by NaOH and GA treatment (P<0.01), which suggested that the intrinsic inhibitors was another factor of seed dormance. So it was considered that the dormance of H. elliptica seed was the comprehensive dormance of the seed coat and inhibitors contained in the seeds. 3) The final germination rates of the seeds treated by different methods were significantly improved, among them, seeds soaked with water by 2 d can make the coat swell, constantly absorbing the water needed for germination; seeds soaked with 30% NaOH by 80 min and then imposed 200 μg/g GA, the effect was the best when the temperature was 25℃, germination rate reached (74.80%±1.71)%.
Key words: ecology; Halenia elliptica; dormancy mechanism; germination rate; dormancy breaking
发表期数: 2016年5月第9期
引用格式: 陈荣健,封 烁,虞红强,等. 椭圆叶花锚种子休眠机制及其破除方法的研究[J]. 中国科技论文在线精品论文,2016,9(9):893-900.
 
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