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‘琼瑶浆’葡萄萜烯糖基转移酶基因的克隆及生物信息学分析

发表时间:2016-05-15  浏览量:1674  下载量:502
全部作者: 问亚琴,李相怡,潘秋红
作者单位: 中国农业大学食品科学与营养工程学院葡萄与葡萄酒研究中心
摘 要: 本研究从酿酒葡萄‘琼瑶浆’果实中克隆得到8个潜在的萜烯糖基转移酶基因,并对其序列进行生物信息学分析。结果显示,克隆得到的8个糖基转移酶基因均具有完整的基因编码框,可以翻译成完整蛋白,且都具有催化糖基转移的保守氨基酸区域PSPG基元;进化树分析表明,这些基因之间相似性为63.2%~76.1%,它们与拟南芥的同源性为49.4%~59.3%. 该研究为后续的基因功能鉴定奠定了基础。
关 键 词: 园艺学;萜烯糖基转移酶;基因序列;葡萄果实
Title: Cloning and bioinformatics analysis of terpene glucosyltransferase in Vitis vinifera L. ‘Gewurztraminer’
Author: WEN Yaqin, LI Xiangyi, PAN Qiuhong
Organization: Center for Viticulture & Enology, College of Food Science & Nutritional Engneering
Abstract: This study aimed at the cloning of eight putative terpene glycosyltransferase genes (UGTs) from the berries of Vitis vinifera L. ‘Gewurztraminer’ and the bioinformatics analysis of these genes. The results showed that the eight UGTs all possessed complete open reading frame and glycosyltransferase-based conservative amino acid area PSPG primitives that could be translated to produce the complete proteins. Evolutionary tree analysis revealed that the sequence similarity between these genes reached 63.2%-76.1%, and the homology of these genes with Arabidopsis glucosyltransferases was within the range of 49.4%-59.3%. This work lays a foundation for subsequent function identification of these genes.
Key words: horticulture; terpene glucosyltransferase; gene sequence; grape berry
发表期数: 2016年5月第9期
引用格式: 问亚琴,李相怡,潘秋红. ‘琼瑶浆’葡萄萜烯糖基转移酶基因的克隆及生物信息学分析[J]. 中国科技论文在线精品论文,2016,9(9):925-932.
 
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