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6个鸭茅品种微卫星遗传变异分析

发表时间:2016-05-15  浏览量:1441  下载量:482
全部作者: 蒋林峰
作者单位: 垫江县畜牧生产站
摘 要: 基于6个供试鸭茅品种,以微卫星标记为手段,对其开展品种内及品种间遗传变异及亲缘关系分析。结果表明:1)6个供试鸭茅品种间平均遗传距离0.143 7,低于0.15,65.30%遗传变异存在于品种内,种质间遗传变异34.70%;2)微卫星标记对6个鸭茅品种150个单株分析表明,15对引物共扩增114条清晰条带,平均每对引物7.6条,多态位点百分率(percentage of polymorphic loci,PPB)100%,多态信息含量(polymorphism information content,PIC)0.4~0.5,平均为0.46. 研究证明,微卫星标记可用于鸭茅品种遗传变异分析,在亲缘关系鉴定上具有重要参考价值。
关 键 词: 草原学;鸭茅;品种;微卫星;遗传变异
Title: Genetic variation in six orchardgrass (Dactylis glomerata L.) cultivars based on microsatellite analysis
Author: JIANG Linfeng
Organization: Department of Livestock Production Station of Dianjiang County
Abstract: In this study, microsatellite analysis was employed to investigate genetic variation and relationships among and within six orchardgrass cultivars. Results indicated that: 1) The average number of genetic distance among six Dactylis glomerata L. cultivars was 0.143 7, below 0.15, and 65.30% of the genetic diversity existing within analysis cultivars, while genetic difference among cultivars was 34.70%. 2) 114 clear bands were amplified by 15 microsatellite primers, based on 150 individual plants from six D. glomerata L. cultivars, the average number of amplified bands by each pair of primer was 7.6, and the percentage of polymorphic loci was 100%. Polymorphism information content (PIC) ranged from 0.4 to 0.5 with an average of 0.46. These results suggested that microsatellite markers had a wide prospective in genetic variation analysis of orchardgrass, and the results had important reference values for D. glomerata L. cultivars.
Key words: range science; Dactylis glomerata L.; cultivars; microsatellite; genetic variation
发表期数: 2016年5月第9期
引用格式: 蒋林峰. 6个鸭茅品种微卫星遗传变异分析[J]. 中国科技论文在线精品论文,2016,9(9):942-948.
 
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