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乳腺炎奶牛整合素α4β7表达的检测

发表时间:2016-05-15  浏览量:1635  下载量:449
全部作者: 渠诗慧,王海霞,刘 辉,刘文博,张乃生
作者单位: 吉林大学动物医学学院;黑龙江省大庆市畜牧兽医局;黑龙江省大庆市动物卫生监督所;黑龙江省大庆市红岗区杏树岗镇人民政府
摘 要: 奶牛乳腺炎是严重危害奶牛养殖业的重大疾病之一,在全球造成了重大的经济损失。乳腺是奶牛的重要器官,其中的淋巴细胞不仅能够对抗病原菌侵袭,还能通过乳汁给幼畜提供被动免疫。有研究表明,淋巴细胞的迁移具有组织特异性,其依赖于细胞表面和血管内皮上粘附分子的表达。为研究淋巴细胞归巢受体α4β7在健康及乳腺炎奶牛中的表达差异,分别取健康及乳腺炎奶牛的血液和乳汁,利用流式细胞术对其中表达α4β7的阳性淋巴细胞进行测定。结果表明,两组奶牛的血液和乳汁中淋巴细胞表面均表达α4β7,乳腺炎奶牛血液及乳汁中表达α4β7细胞的阳性率高于健康组。
关 键 词: 临床兽医学;乳腺炎;淋巴细胞;α4β7
Title: The detection of integrin α4β7 in mastitis cow
Author: QU Shihui, WANG Haixia, LIU Hui, LIU Wenbo, ZHANG Naisheng
Organization: College of Veterinary Medicine, Jilin University; Daqing Animal Husbandry and Veterinary Bureau; Daqing Honggang District Animal Health Supervision; People’s Government of Daqing Honggang District Xingshugang Town
Abstract: Bovine mastitis is one of the major diseases which cause serious economic loss to the dairy farming worldwide. Mammary gland is an important organ to the cow, the lymphocyte can not only fight against pathogens, but also provide assistance from a passive immunization children through breast milk. Research has shown that the migration of lymphocyte has tissue specificity, which depends on the expression of adhesion molecules on the vascular endothelium and cell surface. To further research the differential expression of lymphocytes homing receptor α4β7 in healthy and mastitis bovine, we took blood and milk from healthy and mastitis bovine, respectively, and used flow cytometry to detect lymphocytes which positive express α4β7. The results showed that α4β7 was expressed on lymphocytes both in blood and milk from healthy and mastitis bovine, and the positive rate in mastitis bovine was higher than the healthy bovine.
Key words: clinical veterinary medicine; mastitis; lymphocytes; α4β7
发表期数: 2016年5月第9期
引用格式: 渠诗慧,王海霞,刘 辉,等. 乳腺炎奶牛整合素α4β7表达的检测[J]. 中国科技论文在线精品论文,2016,9(9):955-960.
 
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