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空肠弯曲杆菌Cj0448c缺失突变体的构建

发表时间:2016-05-15  浏览量:1509  下载量:563
全部作者: 郝海红,胡 博,刘振利,袁宗辉
作者单位: 华中农业大学动物医学学院
摘 要: 空肠弯曲杆菌(Campylobacter jejuni)是一种广泛存在的人畜共患的病原菌,其耐药性问题引起了世界的广泛关注。前期研究通过基因芯片方法,发现一种假定的信号转导基因Cj0448c在中高水平大环内酯类耐药空肠弯曲杆菌中下调表达。为探究Cj0448c对空肠弯曲杆菌耐药性和毒力的影响,以C. jejuni NCTC1168为空白菌,首先应用体外诱导方法,获得了大环内酯类耐药突变菌;再应用插入失活技术成功构建了Cj0448c的缺失突变体。本研究将为进一步验证该基因的功能和分析该基因的调控机制奠定基础。
关 键 词: 兽医药理学;空肠弯曲杆菌;耐药性;Cj0448c;基因敲除
Title: Establishment of Cj0448c mutant in Campylobacter jejuni
Author: HAO Haihong, HU Bo, LIU Zhenli, YUAN Zonghui
Organization: College of Veterinary Medicine, Huazhong Agricultural University
Abstract: Campylobacter jejuni is one of a leading zoonotic pathogen. The antibiotic resistance of this pathogen has raised the world concern. In the previous study, the microarray assay indicated that Cj0448c was down-regulated in the intermediate-to-high level of macrolide resistant C. jejuni. The aim of this study was to investigate the role of this gene on the resistance and virulence. In the present study, C. jejuni NCTC1168 was used as the reference strain, the macrolide resistant mutant was obtained by exposure of the reference strain to increased concentration of erythromycin. The Cj0448c deletion mutant was constructed via insertional inactivation. The result of this study can provide a basis for further study on the function and regulation mechanism of this gene.
Key words: veterinary pharmacology; Campylobacter jejuni; antibiotics resistance; Cj0448c; gene knock out
发表期数: 2016年5月第9期
引用格式: 郝海红,胡 博,刘振利,等. 空肠弯曲杆菌Cj0448c缺失突变体的构建[J]. 中国科技论文在线精品论文,2016,9(9):961-967.
 
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