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钩端螺旋体对数生长期及毒力测定

发表时间:2016-05-15  浏览量:1453  下载量:520
全部作者: 谢旭峰,张文龙,曹永国
作者单位: 吉林大学动物医学学院
摘 要: 目的:了解钩端螺旋体的生长规律和毒力,为下一步研究做准备。方法:使用EMJH液体培养基,在28~30℃条件下振荡培养,每隔6 h用细菌计数板检测钩体的浓度;取4~6周龄的雌性仓鼠,每组4只,腹腔注射不同浓度对数生长期的钩体,记录仓鼠的半数死亡天数;另取3组仓鼠,腹腔注射106对数生长期的钩体,检测钩体临4菌株的全致死量,并取感染仓鼠的肾脏、肝脏和肺脏,进行组织病理学观察。结果:1)钩体在0~150 h内呈S型增长,其中0~60 h内生长缓慢,80~105 h内生长迅速,130~150 h则进入稳定期。2)腹腔注射102~105钩体的仓鼠都有半数死亡,但都没有出现全致死,而腹腔注射106钩体的3组仓鼠,都在5~7 d出现了稳定的100%死亡率。3)组织病理学观察感染后的仓鼠肾小管和肺出血明显,肝细胞紧密连接增宽。结论:钩体临4菌株在EMJH培养基,28~30℃条件下培养,对数生长期为80~105 h;该菌株在4~6周龄仓鼠上的半数致死量为<102,全致死量为106,感染仓鼠的肾脏、肝脏和肺脏都出现明显的病理学损伤。
关 键 词: 家畜传染学;钩端螺旋体;对数生长期;半数致死量;病理损伤
Title: Measurement of logarithmic phase and toxicity in Leptospira
Author: XIE Xufeng, ZHANG Wenlong, CAO Yongguo
Organization: College of Veterinary Medicine, Jilin University
Abstract: Objective: To explore the growth characteristics and toxicity of leptospira for the next research. Methods: Leptospira was incubated in EMJH medium using a shaker at 28-30℃ and used bacteria count plate and dark-field microscope to measure the viable count every 6 h. Being chosed 4 groups of 4 to 6-week-old female golden hamsters, each group having four hamsters. Intraperitoneal challenge of hamster with different concentrations’ leptospiroses, keeping a record of the days these hamsters died in half. Besides, being chosed 3 groups of hamsters, intraperitoneally challenged 106 leptospiroses living in logarithmic phase, measuring the lethal dose and geted the infected hamsters’ kidneys, livers and lungs to do histopathological examination. Results: 1) During 0-150 h, Leptospira growth showed S curve. During 0 to 60 h, it growed slowly, developed rapidly in 80-105 h but in 130-150 h came into stable phase. 2) Intraperitoneally challenging hamsters of 102-105 Leptospira all died an half but 3 groups of hamsters intraperitoneally challenged 106 all died in 5-7 d. 3) Histopathological examination showed that infected hamsters’ lungs bled obviously, livers’ cells tight coupling broadened, kidney tubules also bled. Conclusion: The logarithmic phase of Leptospira was 80-105 h in EMJH medium at 28-30℃. The medium lethal dose of Leptospira in 4 to 6-week-old female golden hamster was 102, lethal dose was 106. Infected hamsters showed obviously pathological damage.
Key words: animal molysmology; Leptospira; logarithmic phase; LD50; pathology
发表期数: 2016年5月第9期
引用格式: 谢旭峰,张文龙,曹永国. 钩端螺旋体对数生长期及毒力测定[J]. 中国科技论文在线精品论文,2016,9(9):968-973.
 
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