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HPLC-DAD法同时测定抗感颗粒中15个活性成分含量
发表时间：2016-06-15 浏览量：2302 下载量：846
| 全部作者： | 许春芳，林新强，谢心月，许文，黄鸣清 |
| 作者单位： | 福建中医药大学药学院 |
| 摘 要： | 建立一种高效液相色谱-光电二极管阵列检测器法（high performance liquid chromatography-diode array detector，HPLC-DAD）同时测定抗感颗粒中15种活性成分（没食子酸、新绿原酸、原儿茶酸、对羟基苯甲酸、氧化芍药苷、绿原酸、獐牙菜苷、隐绿原酸、芍药内酯苷、芍药苷、芦丁、五没食子酰葡萄糖、异绿原酸B、异绿原酸A、异绿原酸C）的含量，以Ultimate C18（150 mm × 4.6 mm，5 μm）为色谱柱；以乙腈（B）-0.1%甲酸水（A）为流动相，梯度洗脱；流速：0.8 mL/min；柱温：25℃；进样量：10 μL. 结果表明，HPLC-DAD定量分析抗感颗粒中15种成分在考察的浓度范围内，与峰面积之间呈良好的线性关系（r>0.999 0）；回收率均在95.70%～104.74%范围内，相对标准偏差（relative standard deviation，RSD）在1.90%～4.96%．所建立的HPLC-DAD同时测定抗感颗粒中15种活性成分的定量分析方法简便、快捷、准确，为综合评价抗感制剂的质量提供参考。 |
| 关 键 词： | 中药学；抗感颗粒；HPLC-DAD；质量控制 |
| Title： | Simultaneous determination of fifteen active contents in Kanggan granules by HPLC-DAD |
| Author： | XU Chunfang, LIN Xinqiang, XIE Xinyue, XU Wen, HUANG Mingqing |
| Organization： | School of Pharmacy, Fujian University of Traditional Chinese Medicine |
| Abstract： | In this study, a high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous determination of 15 active contents in Kanggan granules, which are gallic acid, neochlorogenic acid, protocatechuic acid, 4-hydroxybenzoic acid, oxypaeoniflora, chlorogenic acid, swertiamarin, cryptochlorogenin acid, albiflorin, paeoniflorin, rutin, pentagalloylglucose, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C. The 15 active compounds were simultaneously separated by a Ultimate C18 (150 mm × 4.6 mm, 5 μm) column with acetonitrile (B)-0.1% formic acid (A) as the mobile phase and a gradient elution mode set with 0.8 mL/min of flow rate, 25℃ of column temperature and 10 μL of injection volume. The results showed that HPLC-DAD quantitative methodology was validated. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r>0.999 0), and the overall recoveries were ranged from 95.70%-104.74% with the relative standard deviation (RSD) ranging from 1.90%-4.96%. It is the first report about simultaneous analysis of 15 major components in Kanggan granules by using HPLC-DAD method, which is a simple, specific, fast and accurate method for quality control of Kanggan granules. |
| Key words： | science of Chinese pharmacology; Kanggan granules; HPLC-DAD; quality control |
| 发表期数： | 2016年6月第11期 |
| 引用格式： | 许春芳，林新强，谢心月，等. HPLC-DAD法同时测定抗感颗粒中15个活性成分含量[J]. 中国科技论文在线精品论文，2016，9（11）：1081-1089. |
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