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HPLC-DAD法同时测定抗感颗粒中15个活性成分含量

发表时间:2016-06-15  浏览量:1858  下载量:672
全部作者: 许春芳,林新强,谢心月,许文,黄鸣清
作者单位: 福建中医药大学药学院
摘 要: 建立一种高效液相色谱-光电二极管阵列检测器法(high performance liquid chromatography-diode array detector,HPLC-DAD)同时测定抗感颗粒中15种活性成分(没食子酸、新绿原酸、原儿茶酸、对羟基苯甲酸、氧化芍药苷、绿原酸、獐牙菜苷、隐绿原酸、芍药内酯苷、芍药苷、芦丁、五没食子酰葡萄糖、异绿原酸B、异绿原酸A、异绿原酸C)的含量,以Ultimate C18(150 mm × 4.6 mm,5 μm)为色谱柱;以乙腈(B)-0.1%甲酸水(A)为流动相,梯度洗脱;流速:0.8 mL/min;柱温:25℃;进样量:10 μL. 结果表明,HPLC-DAD定量分析抗感颗粒中15种成分在考察的浓度范围内,与峰面积之间呈良好的线性关系(r>0.999 0);回收率均在95.70%~104.74%范围内,相对标准偏差(relative standard deviation,RSD)在1.90%~4.96%.所建立的HPLC-DAD同时测定抗感颗粒中15种活性成分的定量分析方法简便、快捷、准确,为综合评价抗感制剂的质量提供参考。
关 键 词: 中药学;抗感颗粒;HPLC-DAD;质量控制
Title: Simultaneous determination of fifteen active contents in Kanggan granules by HPLC-DAD
Author: XU Chunfang, LIN Xinqiang, XIE Xinyue, XU Wen, HUANG Mingqing
Organization: School of Pharmacy, Fujian University of Traditional Chinese Medicine
Abstract: In this study, a high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous determination of 15 active contents in Kanggan granules, which are gallic acid, neochlorogenic acid, protocatechuic acid, 4-hydroxybenzoic acid, oxypaeoniflora, chlorogenic acid, swertiamarin, cryptochlorogenin acid, albiflorin, paeoniflorin, rutin, pentagalloylglucose, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C. The 15 active compounds were simultaneously separated by a Ultimate C18 (150 mm × 4.6 mm, 5 μm) column with acetonitrile (B)-0.1% formic acid (A) as the mobile phase and a gradient elution mode set with 0.8 mL/min of flow rate, 25℃ of column temperature and 10 μL of injection volume. The results showed that HPLC-DAD quantitative methodology was validated. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r>0.999 0), and the overall recoveries were ranged from 95.70%-104.74% with the relative standard deviation (RSD) ranging from 1.90%-4.96%. It is the first report about simultaneous analysis of 15 major components in Kanggan granules by using HPLC-DAD method, which is a simple, specific, fast and accurate method for quality control of Kanggan granules.
Key words: science of Chinese pharmacology; Kanggan granules; HPLC-DAD; quality control
发表期数: 2016年6月第11期
引用格式: 许春芳,林新强,谢心月,等. HPLC-DAD法同时测定抗感颗粒中15个活性成分含量[J]. 中国科技论文在线精品论文,2016,9(11):1081-1089.
 
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