您的位置:首页  > 论文页面

麦冬多糖调控肥胖小鼠脂质代谢紊乱的代谢组学研究

发表时间:2016-06-15  浏览量:1842  下载量:667
全部作者: 王旭,朱云云,石林林,王源,冯怡
作者单位: 上海中医药大学中药现代制剂技术教育部工程研究中心
摘 要: 目的:基于代谢组学手段探究麦冬多糖MDG-1干预膳食诱导肥胖(diet-induced obesity,DIO)小鼠脂质代谢紊乱可能的作用机制。方法:36只雄性C57BL/6小鼠按体重随机分成空白组、模型组和MDG-1干预组,实验持续16周,于实验终点收集各组小鼠肝组织样本。对肝组织进行超高效液相色谱-飞行时间质谱联用(ultra-performance liquid chromatography/time-of-flight mass spectrometry,UPLC/TOF-MS)的代谢组学分析,并采用偏最小二乘判别分析法(partial least squares-discriminatory analysis,PLS-DA)对数据进行分析。同时,测定体重、累计摄食量、血脂水平等生化指标。结果:与模型组相比,MDG-1可有效控制肥胖小鼠体重增加,改善血脂水平。此外,MDG-1对肥胖小鼠肝组织代谢轮廓具有一定的回调作用,并发现了13个潜在的生物标记物。结论:麦冬多糖MDG-1可以改善肥胖小鼠代谢紊乱,其作用机制可能与减少肝脏中胆固醇及胆汁酸的生物合成、增强酮体的生成有关。
关 键 词: 药理学;麦冬多糖MDG-1;脂质代谢紊乱;UPLC/TOF-MS;生物标记物
Title: Metabonomic study of MDG-1 on regulation of the lipid meta-bolism disorders in fat mice
Author: WANG Xu, ZHU Yunyun, SHI Linlin, WANG Yuan, FENG Yi
Organization: Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine
Abstract: Objective: The research established a metabonomics-based method to investigate underlying mechanism of MDG-1 in preventing lipid metabolism disorders of fat mice with diet-induced obesity (DIO). Methods: 36 male C57BL/6 mice were randomly divided into three groups (control group, model group and MDG-1 intervened group). After 16-weeks treatment, liver samples were analyzed by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC/TOF-MS), to find out the potential identification biomarkers by partial least squares-discriminatory analysis (PLS-DA). In addition, biochemical indices of body weight, food intake and blood lipid levels were collected. Results: Compared with model group, significant weight loss and improved lipid levels in MDG-1 intervened group were achieved. In addition, hepatic metabonomic results exhibited that MDG-1 could reverse abnormally expressed metabolites. Meanwhile, 13 potential biomarkers were discovered. Conclusion: MDG-1 could regulate metabolism disorders in DIO model group. Furthermore, the effect may be realized via restricting the biosynthesis of cholesterol as well as some secondary bile acids, and promoting the generation of ketone body.
Key words: pharmacology; MDG-1 from Ophiopogon japonicus; metabolism disorders; UPLC/TOF-MS; biomarker
发表期数: 2016年6月第11期
引用格式: 王旭,朱云云,石林林,等. 麦冬多糖调控肥胖小鼠脂质代谢紊乱的代谢组学研究[J]. 中国科技论文在线精品论文,2016,9(11):1101-1108.
 
3 评论数 0
暂无评论
友情链接