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薯蓣皂苷对db/db小鼠非酒精性脂肪肝模型糖脂代谢和肝损伤的影响
发表时间：2016-06-15 浏览量：2385 下载量：1011
| 全部作者： | 许丽娜，尹连红，齐艳，彭金咏 |
| 作者单位： | 大连医科大学药学院 |
| 摘 要： | 目的：检测薯蓣皂苷（dioscin，DIO）对非酒精性脂肪肝（nonalcoholic fatty liver disease，NAFLD）db/db小鼠模型的糖脂代谢和胰岛素抵抗相关指标的影响，以评价薯蓣皂苷抗非酒精性脂肪肝作用。方法：db/db小鼠随机分为对照组、模型组、薯蓣皂苷给药组（DIO组）。模型组和DIO组用蛋氨酸胆碱缺乏（methionine-choline deficient，MCD）饲料诱导小鼠形成非酒精性脂肪肝。DIO组每天DIO灌胃80 mg/kg，连续给药4周后，口服糖耐量实验（oral glucose tolerance test，OGTT）检测小鼠糖耐量和胰岛素抵抗的改变情况，测定小鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、总甘油三酯（triglyceride，TG）、总胆固醇（total cholesterol，TC）、血糖（blood glucose，BG）和胰岛素的含量，HE染色和苏丹三染色观察小鼠肝脏组织形态学改变。结果：与模型组比较，DIO组小鼠OGTT中曲线下面积（area under the curve，AUC）显著降低，且血清中ALT、AST、TG、TC、BG值和胰岛素含量明显下降。组织形态学检测发现，模型组小鼠肝脏组织出现脂肪空泡和脂滴蓄积，DIO组显著降低了小鼠肝脏组织的脂肪变性。结论：DIO能明显改善db/db小鼠非酒精性脂肪肝的肝损伤和糖脂质代谢紊乱。 |
| 关 键 词： | 药效学；薯蓣皂苷；非酒精性脂肪肝；db/db小鼠；脂代谢；糖代谢 |
| Title： | Effects of dioscin on lipid metabolism, glucose metabolism and liver injury in db/db mice with nonalcoholic fatty liver disease |
| Author： | XU Lina, YIN Lianhong, QI Yan, PENG Jinyong |
| Organization： | College of Pharmacy, Dalian Medical University |
| Abstract： | Objective: The db/db mice model of nonalcoholic fatty liver disease (NAFLD) was established to investigate the protective effects of dioscin (DIO) on NAFLD. Methods: The mice were randomly distributed into control group, model group and DIO group. Mice in model and DIO group were fed a diet deficient in methionine-choline deficient (MCD) to induce NAFLD. In DIO group, DIO was administered intragastrically (i.g.) at the dose of 80 mg/(kg•d) for four consecutive weeks. Oral glucose tolerance test (OGTT) was carried on for the quantification of the oral glucose tolerance and insulin resistant, and ALT, AST, triglyceride (TG), total cholesterol (TC), blood glucose (BG) and insulin were all detected. At the same time, HE and Sudan III staining were performed to investigate the morphological changes. Results: Compared with mode group, DIO can decrease area under the curve (AUC) in OGTT, and the levels of ALT, AST, TC, TG, BG and insulin were also significantly decreased compared with model group. Morphology results found that fat vacuoles and lipid droplets accumulate appeared in the liver tissue of model group, while the liver steatosis was significantly reduced by DIO adiminstration in DIO group. Conclusion: DIO can significantly improve the liver injury, glucose and lipid metabilism disorder in db/db mice with NAFLD. |
| Key words： | pharmacodynamics; dioscin; nonalcoholic fatty liver disease; db/db mice; lipid metabolism; glucose metabolism |
| 发表期数： | 2016年6月第11期 |
| 引用格式： | 许丽娜，尹连红，齐艳，等. 薯蓣皂苷对db/db小鼠非酒精性脂肪肝模型糖脂代谢和肝损伤的影响[J]. 中国科技论文在线精品论文，2016，9（11）：1109-1114. |
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