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CIP2A抑制非小细胞肺癌细胞凋亡

发表时间:2016-06-15  浏览量:1654  下载量:803
全部作者: 董千泽,付琳,韩勇,刑济麟
作者单位: 中国医科大学基础医学院
摘 要: 目的:探讨蛋白磷酸酶2A的癌性抑制因子(cancerous inhibitor of protein phosphatase 2A,CIP2A)是否上调细胞凋亡蛋白Bcl-2和Bcl-xl的表达。方法:选取HBE细胞系及A549细胞系进行实验,通过流式细胞术、实时PCR(real-time PCR,RT-PCR)和Western blotting方法检测细胞凋亡情况及凋亡相关蛋白Bcl-2、Bcl-xl的表达情况。结果:在HBE细胞中转染CIP2A,A549细胞中干扰CIP2A,发现过表达CIP2A能够显著抑制肺癌细胞凋亡并上调Bcl-2和Bcl-xl的表达,而敲除A549细胞系中CIP2A则促进细胞凋亡并抑制Bcl-2和Bcl-xl的表达。结论:CIP2A通过调节Bcl-2和Bcl-xl的表达进而影响细胞凋亡。
关 键 词: 病理学;非小细胞肺癌;CIP2A;Bcl-2;Bcl-xl;细胞凋亡
Title: CIP2A inhibits cell apoptosis in non-small-cell lung cancer
Author: DONG Qianze, FU Lin, HAN Yong, XING Jilin
Organization: College of Basic Medical Science, China Medical University
Abstract: Objective: To investigate whether cancerous inhibitor of protein phosphatase 2A (CIP2A) upregulates the expression of cell apoptosis protein Bcl-2 and Bcl-xl. Methods: HBE cell lines and A549 cell lines were used for assays. Cell apoptosis and the expression of Bcl-2 and Bcl-xl were detected using flow cytometry, real-time PCR (RT-PCR) and Western blotting. Results: HBE cell lines were transfected with CIP2A, and A549 cell lines were interfered with CIP2A. Overexpression of CIP2A in HBE cell lines can significantly inhibit cell apoptosis of non-small-cell lung cancer and upregulate the expression of Bcl-2 and Bcl-xl at both protein and mRNA level, whereas, knockdown of CIP2A in A549 cell lines can increase cells apoptosis and inhibit the expression of Bcl-2 and Bcl-xl. Conclusion: CIP2A can regulate the expression of Bcl-2 and Bcl-xl to inhibit cells apoptosis.
Key words: pathology; non-small-cell lung cancer; CIP2A; Bcl-2; Bcl-xl; cell apoptosis
发表期数: 2016年6月第11期
引用格式: 董千泽,付琳,韩勇,等. CIP2A抑制非小细胞肺癌细胞凋亡[J]. 中国科技论文在线精品论文,2016,9(11):1136-1141.
 
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