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乙烯信号在鞘脂合成途径中的作用

发表时间:2016-08-15  浏览量:1325  下载量:500
全部作者: 吴建新,吴嘉莉,尹 健,姚 楠
作者单位: 中山大学生命科学学院
摘 要: 鞘脂(sphingolipid)是植物和其他真核生物内膜系统的重要成分,但目前关于鞘脂合成与植物激素间的交互作用知之甚少。前期研究表明,伏马毒素(Fumonisin B1,FB1)抑制神经酰胺合成酶,破坏植物体内的鞘脂平衡。本研究利用FB1处理拟南芥植株,分析比较了乙烯信号途径突变体在表型及鞘脂含量等方面的差异,发现FB1处理后,ein2等乙烯途径受阻突变体表现出对FB1显著的敏感性,而ctr1-1等乙烯持续表达突变体则表现出对FB1较强的抗性。鞘脂成分检测发现,在FB1处理后,神经酰胺以及长链基团(long chain base,LCB)在ein2中积累程度显著高于野生型,而在ctr1-1中的含量则显著低于野生型。此外,在乙烯前体ACC处理能够显著增强拟南芥幼苗对FB1的耐受性,且抑制FB1引起的鞘脂积累。综合以上结果,说明乙烯对调节鞘脂合成具有重要作用。
关 键 词: 植物免疫学;抗性生理;鞘脂合成;乙烯;伏马菌素B1
Title: Role of ethylene signaling on sphingolipid synthesis
Author: WU Jianxin, WU Jiali, YIN Jian, YAO Nan
Organization: School of Life Sciences, Sun Yat-sen University
Abstract: Sphingolipids are primary endomembrane components in plants and other eukaryotes. However, little is known about the cross-talk between sphingolipid biosynthesis and plant hormone. Previous studies indicate that the fungal toxin Fumonisin B1 (FB1) inhibits ceramide synthase activity thus disrupting sphingolipid homeostasis. In this report, we analyzed the phenotypes and sphingolipid profiles of mutants in ethylene signaling before and after FB1 treatment. The results showed that the ethylene signaling deficient mutant ein2 was more sensitive to FB1, whereas the ethylene signaling constitutive activate mutant ctr1-1 was more resistance to FB1 than wild type. Sphingolipid profiles analysis showed long chain bases (LCB) and ceramides were much more accumulated in ein2 mutant, but less accumulated in ctr1-1. Moreover, treatment with the ethylene precursor ACC could significantly increase plant resistance to FB1, and antagonized FB1-induced sphingolipid accumulation. We conclude that ethylene plays an important role in sphingolipid synthesis.
Key words: plant immunology; stress physiology; sphingolipid synthesis; ethylene; Fumonisin B1
发表期数: 2016年8月第15期
引用格式: 吴建新,吴嘉莉,尹 健,等. 乙烯信号在鞘脂合成途径中的作用[J]. 中国科技论文在线精品论文,2016,9(15):1514-1520.
 
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