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玉米鞘腐病拮抗菌株C16的分离鉴定与拮抗基因克隆

发表时间:2016-08-15  浏览量:1463  下载量:499
全部作者: 孙志颖,金 贺,郝志敏
作者单位: 河北农业大学真菌毒素与分子植物病理学实验室;河北农业大学生命科学学院
摘 要: 玉米鞘腐病作为玉米生产新病害,其蔓延将会影响玉米产量和品质。从黄瓜根际土壤中成功分离得到了1株对玉米鞘腐病有优良拮抗活性的菌株C16,经形态学、生理生化和16S rDNA分子生物学鉴定,该菌株为枯草芽孢杆菌。PCR成功验证了菌株C16中编码srfAA、srfAB、srfAC、fenA、fenB、ituB的生物合成基因,通过DNA文库的建立和氨基酸序列比对,菌株C16的fenA基因与已报道产制磷脂菌素Plipastatin而不产Fengycin的模式菌株Bacillus subtilis 168差异显著,与产Fengycin并对丝状真菌有较强拮抗活性的枯草芽孢杆菌SG6相似度高。采用了重叠延伸PCR方法成功获得11 kb的基因全长。
关 键 词: 微生物学;玉米鞘腐病;枯草芽孢杆菌;fenA;基因克隆
Title: Isolation, identification of antagonistic bacteria strain C16 to corn sheath rot and cloning of its antagonistic gene
Author: SUN Zhiying, JIN He, HAO Zhimin
Organization: Lab of Mycotoxins and Molecular Plant Pathology, Hebei Agricultural University; College of Life Sciences, Hebei Agricultural University
Abstract: Abstract: As a new disease, the spread of corn sheath rot has made negative effects to the yield and quality of corn. A strain antagonistic to corn sheath rot was successfully obtained from the healthy cucumber rotational rhizosphere soil, named as C16. The morphological, physiological and biochemical characterization and molecular biological 16S rDNA method identified C16 to be Bacillus subtilis. PCR successfully validated antagonistic genes of C16 strain, including srfAA, srfAB, srfAC, fenA, fenB and ituB. By establishment of DNA library and comparison of amino acid sequences, fenA gene in C16 is significantly different from a non-Fengycin-producing whlie Plipastatin-producing strain B. subtilis 168; fenA in C16 is significantly similar with B. subtilis SG6 who has been reported to produce Fengycin and antagonistic to filamentous fungi strongly. Cloning of the full-length 11 kb gene fenA was successful in overlap PCR way.
Key words: microbiology; corn sheath rot; Bacillus subtilis; fenA; gene cloning
发表期数: 2016年8月第15期
引用格式: 孙志颖,金 贺,郝志敏. 玉米鞘腐病拮抗菌株C16的分离鉴定与拮抗基因克隆[J]. 中国科技论文在线精品论文,2016,9(15):1558-1568.
 
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