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牙鲆两种维生素D受体基因克隆、组织表达与结构比较研究

发表时间:2016-08-15  浏览量:1170  下载量:331
全部作者: 王家庆,李振刚,李绍明,韩宗元
作者单位: 沈阳工学院生命工程学院;吉林大学生命科学学院
摘 要: 维生素D受体(vitamin D receptor,VDR)通过激活和调控肠内钙转运基因介导维生素D及其主要活性代谢产物1,25(OH)2D3的相互作用来发挥它的生物学效应。与四足类动物仅存在的一种类型VDR不同,牙鲆鱼存在两种类型的VDR(VDRa和VDRb),对牙鲆的两种类型VDRa和VDRb的cDNA与蛋白序列以及组织表达进行了比较分析。结果表明,牙鲆VDRa与VDRb核酸序列同源性为82%,蛋白序列同源性84%. VDRa与VDRb在皮肤、小肠、腮和脑组织中均表达,其中VDRa在皮肤与VDRb在小肠中的表达量均较低,而在肝脏,VDRa与VDRb均不表达或者表达量相当低,提示两种VDR为不同类型的受体,其分布具有组织特异性;3´-UTR二级结构分析表明两种受体在茎环结构与自由能大小方面存在不同;三级结构分析显示两种受体蛋白空间结构也存在一定差异,阐明这些结构上的差异将有助于进一步研究维生素D受体与维生素D的相互作用,有助于了解1,25(OH)2D3、VDR和钙代谢的关系,从而为阐明牙鲆幼鱼阶段经常发生的骨骼发育畸形机理提供理论基础。
关 键 词: 分子生物学;牙鲆;维生素D受体;基因克隆;组织表达;蛋白结构
Title: Comparative study on gene cloning, tissue expression and structure of two vitamin D receptors in flounder
Author: WANG Jiaqing, LI Zhengang, LI Shaoming, HAN Zongyuan
Organization: College of Life Engineering, Shenyang Institute of Technology; College of Life Science, Jilin University
Abstract: Abstract: The action of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the active form of vitamin D, is mediated by their specific receptor, vitamin D receptor (VDR), which controls the expression of hormonesensitive genes. VDR is an important factor in calcium homeostasis and bone development. To reveal the function of vitamin D and VDR of fish as well as the molecular structure of VDR, it is important to compare different subtype VDR molecules from the flounder. Flounder VDRa and VDRb share 82% identity at the nucleic acid level and 84% identity at the amino acid level. The expression profile analysis results showed that flounder VDRa and VDRb mRNA were both expressed in skin, intestine, gill and brain. In addition, VDRa in skin and VDRb in intestine weakly expressed but both VDRa and VDRb mRNA are undetectable in liver, which suggests that the two kinds of VDR are different types of receptors, and their distribution is tissue specific; Secondary structure analysis in 3´-UTR showed that there was a difference between the stem loop structure and the free energy of the two receptors; Homology modeling results showed that the VDRa and VDRb had similarity in three-dimensional structure but differences still existed. Elucidation of the differences in these structures will help us to further study the interaction between vitamin D receptor and vitamin D, and help to understand the 1,25(OH)2D3, VDR and calcium metabolism, so as to provide theory basis to clarify the mechanism in frequent skeletal malformation in flounder juvenile stage.
Key words: molecular biology; flounder; vitamin D receptor; gene cloning; tissue expression; protein structure
发表期数: 2016年8月第15期
引用格式: 王家庆,李振刚,李绍明,等. 牙鲆两种维生素D受体基因克隆、组织表达与结构比较研究[J]. 中国科技论文在线精品论文,2016,9(15):1569-1576.
 
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