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基于静电纺丝技术的纳米纱和纳米纤维膜的三维多孔支架的制备及性能研究

发表时间:2016-09-15  浏览量:1269  下载量:397
全部作者: 郭煦然,张楷乐,朱同贺,莫秀梅
作者单位: 东华大学化学化工与生物工程学院;上海交通大学附属第六人民医院泌尿外科
摘 要: 初步探索了胶原蛋白(collagen)/聚(乳酸-己内酯)[P(LLA-CL)]二元复合纳米纤维、纳米纱的纺丝制备工艺,应用扫描电子显微镜(scanning electron microscope,SEM)、力学性能测试、水接触角测试,分析观察纳米纤维、纳米纱的表观形貌并对比分析了其亲水性和力学性能。在细胞增殖、免疫荧光和动物实验中重点探究了纳米纤维和纳米纱孔隙率、孔径对两种支架的组织修复性能影响。与纳米纤维相比,纳米纱不仅具有很好的力学性能及亲水性能,还具有利于细胞长入的大孔径及孔隙率。同时,两种支架的体外细胞毒性、体内皮肤组织修复等测试,均显示纳米纱优于纳米纤维支架。
关 键 词: 生物医学工程学;静电纺丝;纳米纱;胶原蛋白;聚(乳酸-己内酯)
Title: Preparation and properties of the nanoyarn and nanofiber membranes which are three-dimensional porous scaffolds based on electrospinning technology
Author: GUO Xuran, ZHANG Kaile, ZHU Tonghe, MO Xiumei
Organization: College of Chemistry & Chemical Engineering and Biotechnology, Donghua University; Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University
Abstract: In this paper, we explored the electrospinning preparation process of collagen/poly(lactic acid-caprolactone) [P(LLA-CL)] composite nanofiber and nanoyarn. The apparent morphology and mechanical properties of the fibers were observed and analyzed by scanning electron microscope (SEM), mechanical testing and the water contact angle measurement. In the cell proliferation, immunofluorescence and animal experiments, we focused on the porosity and pore size of the two kinds of scaffolds on tissue repair performance. Compared to nanofiber, the nanoyarns not only have good mechanical properties and hydrophilic properties, but also have large aperture and porosity for cells to grow into the scaffold. Meanwhile, the cytotoxicity of cells in vitro and skin tissue repaired in vivo of two kinds of scaffolds all showed that the nanoyarn is superior to the nanofiber.
Key words: biomedical engineering; electrospinning; nanoyarn; collagen; poly(lactic acid-caprolactone)
发表期数: 2016年9月第17期
引用格式: 郭煦然,张楷乐,朱同贺,等. 基于静电纺丝技术的纳米纱和纳米纤维膜的三维多孔支架的制备及性能研究[J]. 中国科技论文在线精品论文,2016,9(17):1739-1747.
 
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