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HPLC-ELSD法测定墨旱莲中旱莲苷A与旱莲苷Ⅰ的含量

发表时间:2016-09-15  浏览量:1413  下载量:303
全部作者: 潘 晴,马鲁豫,田景奎
作者单位: 山东省皮肤病医院;山东省医学科学院科研处;浙江大学生物医学工程与仪器科学学院
摘 要: 目的:研究墨旱莲皂苷的质量标准。方法:对5个不同产地的墨旱莲药材进行性状鉴别,测定其水分和浸出物的含量;应用高效液相色谱(high performance liquid chromatography,HPLC)-蒸发光散射检测器(evaporative light-scattering detector,ELSD),建立墨旱莲皂苷含量的测定方法,按照20倍量70%乙醇,回流提取5次,合并提取液,浓缩,测定含量。结果:水分含量符合药典规定;70%乙醇浸出物含量定为不低于20.0%;墨旱莲总皂苷以旱莲苷A与旱莲苷Ι含量之和计,不得低于0.45%. 结论:本研究对墨旱莲皂苷的质量标准做了探讨,为墨旱莲皂苷的进一步开发奠定基础。
关 键 词: 中药化学;墨旱莲皂苷;质量标准;水分;浸出物
Title: Quality standard determination of ecliptasaponin A and ecliptasaonin Ι from Eclipta using HPLC-ELSD
Author: PAN Qing, MA Luyu, TIAN Jingkui
Organization: Shandong Demartological Hospital; Scientific Research Office, Shandong Academy of Medical Science; College of Biomedical Engineering & Instrument Science, Zhejiang University
Abstract: Objective: To research the quality standards for saponin from the Eclipta. Methods: we identified the characteristics of the Fructus ligustri lucidi from 5 different producing areas, and determined their contents of the moisture and the extraction. We also use the high performance liquid chromatography (HPLC)-evaporative light-scattering detector (ELSD) to establish the method of determining the saponin from Eclipta. We applied the twenty times of 70% ethanol to extract total saponin from the 5 different producing areas’ Eclipta 5 times. Then we merged, enriched the extract and determined the total saponin content. Results: The content of moisture is conformed to the Pharmacopoeia of the People’s Republic of China. The content of the 70% ethanol extraction couldn’t be less than 20%. When the content of the saponin from Eclipta is in the equivalent of ecliptasaponin A and ecliptasaonin Ι, the result couldn’t be less than 0.45%. Conclusion: This study replenished the quality standard of the contents of saponin from Eclipta, which laid a foundation for its further development.
Key words: chemistry of Chinese medicine; saponin from Eclipta; quality standard; moisture; extraction
发表期数: 2016年9月第17期
引用格式: 潘 晴,马鲁豫,田景奎. HPLC-ELSD法测定墨旱莲中旱莲苷A与旱莲苷Ⅰ的含量[J]. 中国科技论文在线精品论文,2016,9(17):1810-1814.
 
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