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电子受体对光合细菌光发酵产氢性能的影响

发表时间:2016-09-30  浏览量:1168  下载量:358
全部作者: 杨鸿辉,白 俐
作者单位: 西安交通大学动力工程多相流国家重点实验室
摘 要: 电子受体能够调节光合发酵产氢反应液的氧化还原电势,促进光合细菌对高还原性底物的利用。研究了电子受体二甲基亚砜(DMSO)、NaHCO3、NaNO3以及O2对类球红细菌(Rhodobacter sphaeroides)HY01光合发酵产氢性能的影响规律。结果表明,当分别添加DMSO 4 mmol/L、NaHCO3 2 mmol/L、O2 0.25%时,最大产氢速率分别提高了5.95%、9.04%、7.22%,而产氢量分别提高了14.12%、4.10%、7.30%. 在合适的电子受体添加量条件下,反应液的pH值可稳定在7.50左右,该pH值也是固氮酶产氢的最适pH值。综上所述,适当添加电子受体可以有效地提高光合发酵产氢效率。
关 键 词: 二次能源;能源微生物;光合细菌;光发酵制氢;电子受体
Title: Effects of electron acceptors on photosynthetic hydrogen production
Author: YANG Honghui, BAI Li
Organization: State Key Laboratory of Multiphase Flow in Power Engineering, Xi’an Jiaotong University
Abstract: Electron acceptors could regulate the redox photential of the photofermentation cultures, and therefore promote the utilization of high reducing substrates. In this paper, electron acceptors, including DMSO, NaHCO3, NaNO3 and O2 were studied on their effects on the photofermentative hydrogen production performance of Rhodobacter sphaeroides HY01. When the dose of DMSO, NaHCO3 and O2 was 4 mmol/L, 2 mmol/L, 0.25%, the maximum hydrogen production rate was enhanced by 5.95%, 9.04%, 7.22% respectively, and the maximum hydrogen yield was enhanced by 14.12%, 4.10% and 7.30% respectively. The pH value of the reaction solution can be stabilized at 7.50 in the condition of suitable electron acceptor addition, which is the optimum pH value for the hydrogen production of nitrogenase. The results suggest that the photofermentative hydrogen production performance could be enhanced by adding proper ammount of electron acceptors.
Key words: secondary energy; energy microbe; photosynthetic bacterium; photofermentative hydrogen production; electron acceptor
发表期数: 2016年9月第18期
引用格式: 杨鸿辉,白 俐. 电子受体对光合细菌光发酵产氢性能的影响[J]. 中国科技论文在线精品论文,2016,9(18):1893-1903.
 
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