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液相色谱-电喷雾电离质谱联用技术分析赤霉素类植物激素的方法研究

发表时间:2016-10-15  浏览量:1204  下载量:262
全部作者: 徐林芳,张晓娜,校瑞,张晓婷,牛家华,卢明华
作者单位: 河南大学化学化工学院
摘 要: 建立了一种高效液相色谱-电喷雾电离质谱联用技术(high performance liquid chromatography-electrospray ionization mass spectrometry,HPLC-ESI-MS)分析赤霉素类植物激素的分析方法。该方法使用Hilic C18(2.1 mm× 50 mm,2.6 μm)色谱柱,以甲醇:水(0.01 mol/L乙酸铵)= 80:20(体积比),流速0.2 mL/min条件下分离,采用电喷雾-负离子多反应监测(multiple-reaction monitoring,MRM)模式,外标法定量。选择确定赤霉素GA3、GA12母离子依次为(m/z)345.3,331.4,定量子离子依次为(m/z)143.0、220.9,201.0、269.2. 结果表明:该方法在0.05~10.00 μg/mL范围内有良好的线性关系(R2>0.99),检测限为0.01 μg/mL,在0.20,1.50,8.00 μg/mL 3个添加水平下,平均回收率在80.00%~120.00%之间,相对标准偏差(relative standard deviation,RSD)在0.26%~1.90%之间。
关 键 词: 分析化学;高效液相色谱-电喷雾电离质谱;赤霉素;植物激素;分析方法研究
Title: Analysis of gibberellin class plant hormones by HPLC coupled with ESI-MS
Author: XU Linfang, ZHANG Xiaona, XIAO Rui, ZHANG Xiaoting, NIU Jiahua, LU Minghua
Organization: College of Chemistry and Chemical Engineering, Henan University
Abstract: A high performance liquid chromatography (HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) for the analysis of gibberellins was developed in this research. This method used a Hilic C18 (2.1 mm×50 mm, 2.6 μm) column, methanol:water (0.01 mol/L ammonium acetate)=80:20 (volume ratio), separation of flow rate at 0.2 mL/min, by using electrospray ionization in multiple-reaction monitoring (MRM) mode, external method for quantitative. The parent ions of GA3 and GA12 were at m/z 345.3 and 331.4, and the quantitative daughter ions were at m/z 143.0, 220.9 and 201.0, 269.2, respectively. The results showed that the established method had a good linear relationship (R2>0.99) in the 0.05-10.00 μg/mL linear range, the detection limit was 0.01 μg/mL, and the average recovery rate was between 80.00% and 120.00% in three supplemental level of 0.20, 1.50, 8.00 μg/mL with relative standard deviation (RSD) was between 0.26% and 1.90%.
Key words: analytical chemistry; high performance liquid chromatography-electrospray ionization mass spectrometry; gibberellins; plant hormone; analytical method research
发表期数: 2016年10月第19期
引用格式: 徐林芳,张晓娜,校瑞,等. 液相色谱-电喷雾电离质谱联用技术分析赤霉素类植物激素的方法研究[J]. 中国科技论文在线精品论文,2016,9(19):2035-2040.
 
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