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一个外源Wolbachia株系在新宿主种群中的感染率动态

发表时间:2016-11-15  浏览量:1477  下载量:405
全部作者: 周晓飞,李正西
作者单位: 中国农业大学植物保护学院
摘 要: Wolbachia是母系遗传的立克次氏体菌,可通过人工转染对宿主种群进行控制,但效果不稳定。首先从黑腹果蝇中分离到wMel株系,然后将其转染到烟粉虱中。经过荧光原位杂交(fluorescence in situ hybridization, FISH)技术证实该株系已被成功转染到新宿主中,然后采用特异PCR引物检测该株系在新宿主种群中的感染率。结果表明,经过3代,其可在新宿主中稳定传代,但第 6代后,感染率出现了较大波动。该结果提示,外源Wolbachia或许可较快地在新宿主中定殖,但其对靶标宿主种群的调控可能比预想的更复杂。
关 键 词: 植物保护学;Wolbachia;显微注射;荧光原位杂交;感染率;烟粉虱
Title: Dynamics of the infection rate of an exogenous Wolbachia strain in the new host population
Author: ZHOU Xiaofei, LI Zhengxi
Organization: College of Plant Protection, China Agricultural University
Abstract: Wolbachia are maternally-inherited rickettsia-like bacteria, which can be applied to host population control through transinfection, but the control efficiency is unstable. The wMel Wolbachia strain was firstly isolated from the fruit fly Drosophila melanogaster, and then transferred into the whitefly Bemisia tabaci. Fluorescence in situ hybridization (FISH) demonstrated that the wMel strain was successfully transinfected into the new host. Wolbachia-specific primers were used to estimate the dynamics of the infection rate. The results showed that the strain could be steadily inherited in the new host population after 3 generations, but the infection rate of the strain fluctuated after 6 generations, indicating that the exogenous Wolbachia strain can rapidly establish in the new host, but its regulation of target host population may be a more complicated process than expected.
Key words: plant protection; Wolbachia; microinjection; fluorescence in situ hybridization; infection rate; Bemisia tabaci
发表期数: 2016年11月第21期
引用格式: 周晓飞,李正西. 一个外源Wolbachia株系在新宿主种群中的感染率动态[J]. 中国科技论文在线精品论文,2016,9(21):2207-2210.
 
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