您的位置:首页  > 论文页面

大环内酯类药物对猪链球菌生物被膜群体感应系统的影响

发表时间:2016-11-15  浏览量:1314  下载量:265
全部作者: 周永辉,赵玉林,李艳华
作者单位: 东北农业大学动物医学学院
摘 要: 为确定大环内酯类药物对群体感应(quorum-sensing,QS)系统的影响,通过结晶紫染色发现大环内酯类药物亚抑菌浓度干预猪链球菌生物被膜形成,采用蛋白质组学iTRAQ技术分析差异蛋白,阐明大环内酯类药物可能作用的QS系统蛋白靶点;使用实时定量PCR(quantitative real-time PCR,qRT-PCR)技术对QS系统相关蛋白基因(comAB)进行分析,从转录水平验证QS系统的变化。最终证明大环内酯类药物可以通过影响QS系统而影响生物被膜的形成。
关 键 词: 兽医药理学;猪链球菌生物被膜;群体感应系统;大环内酯类药物;iTRAQ;实时定量PCR
Title: Effect of macrolides with Streptococcus suis biofilm quorum-sensing system
Author: ZHOU Yonghui, ZHAO Yulin, LI Yanhua
Organization: College of Veterinary Medicine, Northeast Agricultural University
Abstract: To determine the effects of macrolides antibiotics on the quorum-sensing (QS) system, the antibiotics of subinhibitory concentration was used to intervented biofilm formation by crystal violet staining. We analyzed the differentially expressed proteins by proteome iTRAQ technology to determine the effects of macrolides on the QS system to find the potential protein targets. The changes of QS system were verified by the transcriptional level (comAB) through quantitative real-time PCR (qRT-PCR) technology. Finally, it is proved that the QS system could affect the formation of biofilm.
Key words: veterinary pharmacology; Streptococcus suis biofilm; quorum-sensing system; macrolides; iTRAQ; quantitative real-time PCR
发表期数: 2016年11月第21期
引用格式: 周永辉,赵玉林,李艳华. 大环内酯类药物对猪链球菌生物被膜群体感应系统的影响[J]. 中国科技论文在线精品论文,2016,9(21):2252-2259.
 
0 评论数 0
暂无评论
友情链接