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超声靶向微泡爆破结合纳米粒提高脑胶质瘤靶向治疗效果

发表时间:2016-12-15  浏览量:1540  下载量:376
全部作者: 杨靖靖,赵应征,鲁翠涛
作者单位: 温州医科大学药学院;温州医科大学附属第二医院
摘 要: 超声靶向微泡爆破(ultrasound-targeted microbubble destruction,UTMD)技术已在药物靶向递送系统中展示出很多优势。研究利用UTMD技术提高西仑吉肽纳米粒的靶向递送效率,实现胶质瘤的靶向治疗。采用新工艺制备明胶-泊洛沙姆的水包水(W/W)型西仑吉肽纳米粒,超声-冷冻干燥法制备脂质微泡。通过观察溶液外观及测定粒径、Zeta电位、包封率等,表征西仑吉肽纳米粒和微泡的各项性能。采用C6细胞评价超声结合西仑吉肽纳米粒的体外抗肿瘤效应。用雄性SD大鼠建立脑胶质瘤模型,HE染色、免疫组化评价超声结合西仑吉肽纳米粒的体内抗肿瘤效果。结果表明,脂质微泡的浓度为2×109/mL,平均粒径为3.26 μm. 西仑吉肽纳米粒形态圆整,平均粒径为(112.00±1.63)nm,Zeta 电位值为-(15.8±0.2)mV,纳米粒包封率为(87.5±1.4)%. 细胞毒性实验、HE染色和免疫组化均证实,UTMD联合西仑吉肽纳米粒能够明显促进脑胶质瘤对药物的摄取,诱导肿瘤的凋亡。总之,UTMD技术结合西仑吉肽纳米粒能明显提高脑胶质瘤靶向治疗效果,未来具有较好的临床应用前景。
关 键 词: 药剂学;脑胶质瘤;纳米技术;超声靶向微泡爆破;西仑吉肽;靶向治疗
Title: UTMD combining with nanoparticles for glioma targeted therapy
Author: YANG Jingjing, ZHAO Yingzheng, LU Cuitao
Organization: School of Pharmaceutical Sciences, Wenzhou Medical University; The Second Affiliated Hospital of Wenzhou Medical University
Abstract: Ultrasound-targeted microbubble destruction (UTMD) technology shows a lot of advantages in drug targeting delivery systems. UTMD technology combined with Cilengitide nanoparticles were used to improve the effect of gliomas targeted therapy. W/W type of Cilengitide-loaded gelatin-poloxamer nanoparticles were prepared by novel methods, and ultrasound-lyophilization method was used to prepare phospholipid-based microbubbles. The characterization of Cilengitide nanoparticles were evaluated by the appearance of the solution, particle size, Zeta potentials and drug encapsulation efficiency. C6 cells were used to evaluate the antitumor effects of Cilengitide nanoparticles combined with UTMD technology in vitro. Glioma models were established by using male SD rats. HE stain and immunohistochemistry were used to evaluate the anti-tumor effect in vivo. The results show that the concentration of phospholipid-based microbubble is 2×109/mL, with an average particle size of 3.26 μm. Cilengitide nanoparticles possess good elliptical morphology and excellent dispersion performance. The average particle size of Cilengitide nanoparticles is (112.00±1.63) nm. The Zeta potential value is -(15.8±0.2) mV. The encapsulation efficiency of Cilengitide nanoparticles reaches (87.5±1.4)%. Cytotoxicity test, HE staining and immunohistochemistry confirm UTMD technology combined with Cilengitide nanoparticles can obviously facilitate uptake of Cilengitide in glioma, and enhance the apoptosis of the tumor in vivo. In conclusion, UTMD technology combined with Cilengitide nanoparticles significantly improves the glioma-targeted therapeutic effect, showing great potentials in clinical application.
Key words: pharmaceutics; gliomas; nano technology; ultrasound-targeted microbubble destruction; Cilengitide; targeted therapy
发表期数: 2016年12月第23期
引用格式: 杨靖靖,赵应征,鲁翠涛. 超声靶向微泡爆破结合纳米粒提高脑胶质瘤靶向治疗效果[J]. 中国科技论文在线精品论文,2016,9(23):2411-2419.
 
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