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多功能碳量子点介导microRNA细胞内示踪与转运的研究

发表时间:2016-12-15  浏览量:1445  下载量:447
全部作者: 张苗苗,赵雪纷,房正绉,牛媛媛,杜凤移,夏圣,娄嘉明
作者单位: 江苏大学医学院
摘 要: microRNA(miRNA)能降解靶基因的mRNA或者抑制靶基因的翻译,具有巨大的基础医学研究与临床治疗应用的潜能,然而尚缺乏兼具胞内示踪与基因转运的多功能基因载体。本研究利用一步水热法制备透明质酸-聚乙烯亚胺功能化碳量子点(hyaluronic acid-polyethyleneimine functionalized carbon dots,HP-CDs),通过透射电子显微镜(transmission electron microscope,TEM)、傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)、荧光光谱、X射线衍射(X-ray diffraction,XRD)光谱对其进行理化表征。结果表明,HP-CDs具有较为均一的粒径分布(~3.2 nm)、丰富的亲水表面基团、稳定的荧光性能和较高的荧光量子产率(13.2%)。细胞实验表明,HP-CDs具有较好的生物相容性、基因转载能力以及介导miRNA-30b转染的能力。进入细胞内部的HP-CDs可发出明亮的荧光,可作为有效的示踪基因载体用于miRNA胞内的可视化研究。
关 键 词: 生物医学工程学;氮掺杂碳量子点;透明质酸;聚乙烯亚胺;基因转运
Title: The study of multifunctional carbon dots for microRNA real-time tracking and delivery
Author: ZHANG Miaomiao, ZHAO Xuefen, FANG Zhengzou, NIU Yuanyuan, DU Fengyi, XIA Sheng, LOU Jiaming
Organization: School of Medicine, Jiangsu University
Abstract: microRNA (miRNA) has great potential in preclinical medicine research and clinical treatment due to they can inhibit target genes expression by transcription level or protein level. However, there is lack of gene carriers which can be used in intracelluar tracking and gene transportation. Hyaluronic acid- polyethyleneimine functionalized carbon dots (HP-CDs) were prepared by one-step hydrothemal method. Transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), fluorescence spectrum, X-ray diffraction (XRD) spectra were used to characterize the physiochemical properties of HP-CDs. The results demonstrated that HP-CDs possessed uniform size distribution (~3.2 nm), a large number of hydrophilic groups, stable fluorescence and high quantum yeild (13.2%). Meanwhile, HP-CDs were endowed with favorable biocompatibility, gene loading and miRNA-30b transfection capacity. HP-CDs which go into cells can emit fluorescence, which make it a kind of effective tracking gene vector for visualization research of miRNA.
Key words: biomedical engineering; nitrogen-doped carbon dots; hyaluronic acid; polyethylenimine; gene transportation
发表期数: 2016年12月第23期
引用格式: 张苗苗,赵雪纷,房正绉,等. 多功能碳量子点介导microRNA细胞内示踪与转运的研究[J]. 中国科技论文在线精品论文,2016,9(23):2420-2425.
 
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