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膜式微流控方法添加/去除低温保护剂的实验研究

发表时间:2016-12-15  浏览量:1522  下载量:489
全部作者: 尹来山,周晓明,汪志琨,花友文,张莹
作者单位: 电子科技大学机械电子工程学院;北京协和医学院&中国医学科学院输血研究所
摘 要: 随着干细胞等材料在临床和科研中的广泛应用,低温保存面临着越来越多的微小体积对象,这就对低温保护剂(cryoprotectant agents,CPAs)的添加/去除工作提出了新的处理要求。研究提出一种基于微流控和膜分离技术的方法,并基于该方法建立了夹膜式微流控系统。实验证明,该方法可实现小体积样品细胞CPAs的平稳、可控添加/去除处理,有望大幅度提高小体积生物样品CPAs处理的效率和效果。
关 键 词: 生物医学工程学;低温保护剂;微流控芯片;膜分离技术
Title: Experimental study of a membrane-based microfluidic approach for adding/removing cryoprotectant agents
Author: YIN Laishan, ZHOU Xiaoming, WANG Zhikun, HUA Youwen, ZHANG Ying
Organization: School of Mechatronics Engineering, University of Electronic Science and Technology of China; Institute of Blood Transfusion, Peking Union Medical College & Chinese Academy of Medical Sciences
Abstract: Along with the wide application of stem cells and other materials in clinical and scientific researches, cryopreservation faces a growing number of small volume objects. Some new requirements have been raised during adding/removing cryoprotectant agents (CPAs) for such small volume objects. In this study, a novel membrane-based microfluidic approach was presented. Accordingly, a membrane-based microfluidic system was designed and fabricated by microfluidics and membrane separation technique. Experiment results demonstrated that the system can achieve stable and controllable adding/removing CPAs for small volume samples, and it will greatly improve the efficiency and effectiveness of adding/removing CPAs for small volume biological samples.
Key words: biomedical engineering; cryoprotectant agents; microfluidic chip; membrane separation technique
发表期数: 2016年12月第23期
引用格式: 尹来山,周晓明,汪志琨,等. 膜式微流控方法添加/去除低温保护剂的实验研究[J]. 中国科技论文在线精品论文,2016,9(23):2426-2432.
 
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