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TRPC1在Visfatin诱导心肌细胞肥大中的作用研究

发表时间:2016-12-15  浏览量:1311  下载量:449
全部作者: 李君丽,赵明月,吴文超,刘小菁
作者单位: 四川大学华西医院再生医学研究中心
摘 要: 目的:通过构建特异性沉默经典瞬时受体电位(transient receptor potential channels,TRPC1)基因表达的质粒,初步探讨TRPC1在Visfatin诱导心肌细胞肥大中的作用及其机制。方法:用Visfatin刺激体外培养的H9c2心肌细胞不同时间后,检测心肌细胞肥大表型与TRPC1表达变化。转染TRPC1-shRNA质粒后再给予Visfatin刺激细胞,或特异性上调TRPC1基因表达后进行肥大指标检测以进行机制探讨。结果:100 µg/L浓度的Visfatin作用24,48,72 h均能诱导心肌细胞出现明显的肥大表型,在此过程中伴随着TRPC1表达的显著上调。成功构建沉默TRPC1基因表达的TRPC1-shRNA质粒并转染细胞48 h后,发现心肌细胞肥大标志物脑钠肽(brain natriuretic peptide,BNP)的水平明显下调,而在转染过表达质粒特异性上调TRPC1表达后BNP的水平明显增高。结论:实验结果提示,TRPC1可能介导了Visfatin诱导的心肌细胞发生的肥大反应。
关 键 词: 医学细胞生物学;TRPC1;Visfatin;心肌细胞肥大
Title: Role of TRPC1 in the cardiomyocyte hypertrophy induced by Visfatin
Author: LI Junli, ZHAO Mingyue, WU Wenchao, LIU Xiaojing
Organization: Regenerative Medicine Research Center, West China Hospital, Sichuan University
Abstract: Objective: The aim of this study is to investigate the role and mechanism of transient receptor potential channels (TRPC1) in the cardiomyocyte hypertrophy induced by Visfatin by constructing the specific gene expression plasmid. Methods: Cultured H9c2 cardiomyocytes were exposed to Visfatin at different times in vitro, and the expression of cardiomyocyte hypertrophy markers and TRPC1 were detected. Moreover, constructed TRPC1-shRNA expressing plasmid was used to transfect the cells before Visfatin stimulation, and the overexpression plasmid of TRPC1 was performed to induce TRPC1 forced expression. Results: The experimental data demonstrated that exposure to 100 µg/L concentration of Visfatin for 24, 48, 72 h significantly increased the expression of marker genes for cardiomyocyte hypertrophy and TRPC1. Furthermore, transfection of specific TRPC1-shRNA for 48 h partially inhibited the Visfatin-induced mRNA expression of brain natriuretic peptide (BNP) in H9c2 cells, whereas overexpression of TRPC1 promoted the level of BNP. Conclusion: The results suggest that TRPC1 might mediate cardiomyocyte hypertrophy induced by Visfatin.
Key words: medical cell biology; TRPC1; Visfatin; cardiomyocyte hypertrophy
发表期数: 2016年12月第23期
引用格式: 李君丽,赵明月,吴文超,等. TRPC1在Visfatin诱导心肌细胞肥大中的作用研究[J]. 中国科技论文在线精品论文,2016,9(23):2433-2439.
 
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